Hsp70 Recombinant Rabbit Monoclonal Antibody [PD01-65]
Catalog# HA721497
Hsp70 Recombinant Rabbit Monoclonal Antibody [PD01-65]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
概述
产品名称
Hsp70 Recombinant Rabbit Monoclonal Antibody [PD01-65]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant fragment within Human Hsp70 aa 50-300.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 70 kDa
阳性对照
HCT 116 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, human breast carcinoma tissue, human liver carcinoma tissue.
偶联
unconjugated
克隆号
PD01-65
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200
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IF-Tissue
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1:200
靶点
功能
The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
背景文献
1. Albakova Z et al. HSP70 Multi-Functionality in Cancer. Cells. 2020 Mar
2. Zhang H et al. Hsp70 in Redox Homeostasis. Cells. 2022 Feb
序列相似性
Belongs to the heat shock protein 70 family.
组织特异性
HSPA1B is testis-specific.
翻译后修饰
In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.
亚细胞定位
Cytoplasm.
UNIPROT
别名
DnaK type molecular chaperone HSP70 1 antibody
Epididymis secretory protein Li 103 antibody
FLJ54303 antibody
FLJ54370 antibody
FLJ54392 antibody
FLJ54408 antibody
FLJ75127 antibody
Heat shock 70 kDa protein 1 antibody
Heat shock 70 kDa protein 1/2 antibody
Heat shock 70 kDa protein 1A/1B antibody
展开图片
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Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (HA721497) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A431 cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721497) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Application: IF-Tissue
Species: Human
Site: breast carcinoma
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Hsp70 antibody (HA721497) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721497) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Hsp70 antibody (HA721497) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721497) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Mechanism of Tau Protein Incorporation into Exosomes via Cooperative Recognition of KFERQ-like Motifs by LAMP2A and HSP70
期刊: Neurochemistry International
DOI: 10.1016/j.neuint.2025.105976
IF: 4.4
应用: WB
反应种属: Human
发表时间: 2025 Apr
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Interplay between ferric uptake regulator Fur and horizontally acquired virulence regulator EsrB coordinates virulence gene expression in Edwardsiella piscicida
期刊: Microbiological Research
DOI:
IF: 6.7
应用: WB
反应种属: Human
发表时间: 2021 Dec
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