EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]
Catalog# HA721323
EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]
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WB
-
IHC-P
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IHC-Fr
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Mouse
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Rat
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Human
预测的种属支持售后
概述
产品名称
EAAT2 Recombinant Rabbit Monoclonal Antibody [PS01-62]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human EAAT2
种属反应性
Mouse, Rat (Predicted: Human)
预测的种属支持售后
验证应用
WB, IHC-P, IHC-Fr
分子量
Predicted band size: 62 kDa
阳性对照
Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.
偶联
unconjugated
克隆号
PS01-62
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:2,000
-
IHC-P
-
1:2,000
-
IHC-Fr
-
1:200-1:500
靶点
功能
Excitatory amino acid transporter 2 (EAAT2) also known as solute carrier family 1 member 2 (SLC1A2) and glutamate transporter 1 (GLT-1) is a protein that in humans is encoded by the SLC1A2 gene. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. SLC1A2 / EAAT2 is a member of a family of the solute carrier family of proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. EAAT2 is responsible for over 90% of glutamate reuptake within the brain.
背景文献
1. Blacker CJ et al. EAAT2 as a Research Target in Bipolar Disorder and Unipolar Depression: A Systematic Review. Mol Neuropsychiatry. 2020 Apr
2. Green JL et al. Role of glutamate excitotoxicity and glutamate transporter EAAT2 in epilepsy: Opportunities for novel therapeutics development. Biochem Pharmacol. 2021 Nov
亚细胞定位
Cell membrane.
别名
EAA2_HUMAN antibody
EAAT2 antibody
Excitatory amino acid transporter 2 antibody
Excitotoxic amino acid transporter 2 antibody
Glial high affinity glutamate transporter antibody
GLT 1 antibody
GLT1 antibody
Glutamate aspartate transporter II antibody
Glutamate transporter 1 antibody
Glutamate/aspartate transporter II antibody
展开图片
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Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/500 dilution.
The section was not undergone antigen retrieval.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721323, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721323, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
☑ Relative expression (RE)
Western blot analysis of EAAT2 on different lysates with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse cerebellum tissue lysate (no heat)
Lane 3: Mouse lung tissue lysate (negative) (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721323) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721323) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EAAT2 antibody (HA721323) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721323) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
EAAT2 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Rat,Mouse
Conjugate: unconjugated
