iFluor™ 488 Conjugated BrdU Recombinant Rabbit Monoclonal Antibody [PSH0-18]

Immunocytochemistry analysis of NIH/3T3 cells (Untreated / Brdu treated) labeling BrdU with Rabbit anti-BrdU antibody (HA720186F) at 1/50 dilution.

Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCL in TBST for 30 minutes at room temperature. permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BrdU antibody (HA720186F, iFluor™ 488) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Immunofluorescence analysis of paraffin-embedded BrdU treated/untreated mouse embryo tissue labeling BrdU with Rabbit anti-BrdU antibody (HA720186F) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720186F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

Dot plot showing BrdU treated HeLa cells stained with HA720186F. Cells were incubated with 10 µM BrdU for 24 hours prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol at 4℃ for 30 minutes. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature and then neutralised with borate buffer (0.1M, pH8.5) for 15 minutes.

Samples were washed and incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (HA720186F, 10µg/ml) for 30 min at room temperature.

PI was added to cells 15 min prior to data acquisition.

Dot plot showing untreated HeLa cells (Negative) stained with HA720186F. Cells were incubated without 10 µM BrdU for 30 minutes prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol at 4℃ for 30 minutes. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature and then neutralised with borate buffer (0.1M, pH8.5) for 15 minutes.

Samples were washed and incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (HA720186F, 10µg/ml) for 30 min at room temperature.

PI was added to cells 15 min prior to data acquisition.