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iFluor™ 647 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

RMB: 3280

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Catalog# HA720144F

iFluor™ 647 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

Application

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

概述

产品名称

iFluor™ 647 Conjugated Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Cytokeratin 7 aa 18-67.

种属反应性

Human, Mouse

验证应用

IF-Cell, IF-Tissue, FC

分子量

Predicted band size: 51 kDa

阳性对照

SK-Br-3, human liver tissue, human breast tissue.

偶联

iFluor™ 647

克隆号

ST50-05

RRID

AB_3073487

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:50-1:200

  • FC

  • 1ug/mL

靶点

功能

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.

背景文献

1. Hrudka J. et al. Cytokeratin 7 expression as a predictor of an unfavorable prognosis in colorectal carcinoma. Sci Rep. 2021 Sep

2. Statz E. et al. Cytokeratin 7, GATA3, and SOX-10 is a Comprehensive Panel in Diagnosing Triple Negative Breast Cancer Brain Metastases. Int J Surg Pathol. 2021 Aug

亚细胞定位

Cytoplasm.

UNIPROT #

SwissProt: P08729 Human

SwissProt: Q9DCV7 Mouse

别名

CK 7 antibody

CK-7 antibody

CK7 antibody

Cytokeratin 7 antibody

Cytokeratin-7 antibody

D15Wsu77e antibody

K2C7 antibody

K2C7_HUMAN antibody

K7 antibody

Keratin 7 antibody

展开

图片

  • Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta III Tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/800 dilution.

    Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (HA720144F) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (HA720144F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

  • Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>) and Cytokeratin 8 (HA720132F).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>, red) at 1/200 dilution and Cytokeratin 8 (<a href="/products/HA720132F" style="font-weight: bold;text-decoration: underline;">HA720132F</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.<br /><br />DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720144F) and Cytokeratin 8 (HA720132F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/200 dilution and Cytokeratin 8 (HA720132F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

    DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>), Cytokeratin 8 (<a href="/products/HA720132F" style="font-weight: bold;text-decoration: underline;">HA720132F</a>) and Vimentin (EM0401).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>, red) at 1/50 dilution, Cytokeratin 8 (<a href="/products/HA720132F" style="font-weight: bold;text-decoration: underline;">HA720132F</a>, green) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />Alexa Fluor&reg; 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F), Cytokeratin 8 (HA720132F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/50 dilution, Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>, iFluor&trade; 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720144F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  • Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (<a href="/products/HA720144F" style="font-weight: bold;text-decoration: underline;">HA720144F</a>, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7.

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720144F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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