PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
Rmb: 5600
Catalog# HA610458
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA601677
含抗保成分
-
unconjugated
概述
产品名称
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric) - BSA and Azide free
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human PARK7 aa 11-60 / 189.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 20 kDa
阳性对照
SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
SN07-21
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
PBS (pH7.4).
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies. Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.
背景文献
1. Marín-Buera L et al. Differential proteomic profiling unveils new molecular mechanisms associated with mitochondrial complex III deficiency. J Proteomics 113:38-56 (2015).
2. Bifsha P et al. Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra. PLoS Genet 10:e1004863 (2014).
亚细胞定位
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus.
别名
CAP1 antibody
DJ-1 antibody
DJ1 antibody
DJ1 protein antibody
Epididymis secretory sperm binding protein Li 67p antibody
FLJ27376 antibody
FLJ34360 antibody
FLJ92274 antibody
HEL S 67p antibody
Oncogene DJ1 antibody
展开图片
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This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Western blot analysis of PARK7/DJ1 on different lysates with Rat anti-PARK7/DJ1 antibody (HA601677) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601677, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 20 kDa
Observed band size: 20 kDa -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature. -
This data was developed using HA601677, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601677, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1123, 20 minutes at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
PARK7/DJ1 Recombinant Antibody [SN07-21] - Rat IgG1 (Chimeric)
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
PARK7/DJ1 Recombinant Rabbit Monoclonal Antibody [SN07-21] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
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