Kappa Light Chain Recombinant Mouse Monoclonal Antibody [A8H10-R] - BSA and Azide free
Catalog# HA610141
Kappa Light Chain Recombinant Mouse Monoclonal Antibody [A8H10-R] - BSA and Azide free
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WB
-
IHC-P
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Human
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HA601266
含抗保成分
-
unconjugated
概述
产品名称
Kappa Light Chain Recombinant Mouse Monoclonal Antibody [A8H10-R] - BSA and Azide free
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant full protein within human IGKC.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 12 kDa
阳性对照
Human plasma lysates, human tonsil tissue, human appendix tissue.
偶联
unconjugated
克隆号
A8H10-R
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:500-1:1,000
靶点
功能
Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda. The gene rearrangement process that generates the immunoglobulin molecule results in either a productive kappa gene or a productive lambda gene. The mechanics of the rearrangement process normally produce approximately twice as many kappa-bearing cells as lambda. However this ratio loses during malignant transformation. The kappa light chain antibody labels kappa light chain expressing B lymphocytes and plasma cells. Other cells may also express kappa light chain due to nonspecific uptake of immunoglobulin. Individual B cells express either kappa or lambda light chains. Monoclonality is generally assumed to be evidence of a malignant proliferation. Paired with lambda, kappa light chain is useful in identifying monoclonality of lymphoid malignancies. Anti-Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, anti-kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulins. When dealing with B-cell neoplasms, the determination of light chain ratios remains the centerpiece. Most B-cell lymphomas express either kappa or lambda light chains, whereas reactive proliferations display a mixture of kappa and lambda positive cells. If only a single light chain type is detected, a lymphoproliferative disorder exists. Monoclonality is determined by a kappa-lambda ratio of greater than or equal to 3:1 or a lambda-kappa ratio greater than 2:1. Tonsil is an appropriate control: Approximately half of the peripheral mantle zone B-cells must show a distinct membranous staining reaction for IgK, while the remaining mantle zone B-cells (which are IgL producing) should be unstained.
背景文献
1. McHeyzer-Williams M., Okitsu S., Wang N., McHeyzer-Williams L. Molecular programming of B cell memory. Nat. Rev. Immunol. 12:24-34 (2012)
2. Jaffe ES et al (Eds) WHO Classification Tumours of Haematopoietic and Lymphoid Tissue. IARC Press 2001. Ashton-Key M, Jessup E, Isaacson PG. Immunoglobulin light chain staining in paraffin-embedded tissue using a heat mediated epitope retrieval method. Histopathology. 1996 Dec;29(6):525-31.
亚细胞定位
Cell membrane, Secreted.
UNIPROT
别名
HCAK 1 antibody
HCAK1 antibody
Ig kappa chain C region antibody
IGKC antibody
IGKCD antibody
Immunoglobulin InV antibody
Immunoglobulin kappa constant antibody
Immunoglobulin kappa constant region antibody
Immunoglobulin kappa light chain antibody
Immunoglobulin kappa light chain constant region antibody
展开图片
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Western blot analysis of Kappa Light Chain on human plasma lysates with Mouse anti-Kappa Light Chain antibody (HA610141) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 25 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Kappa Light Chain antibody (HA610141) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610141) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Kappa Light Chain antibody (HA610141) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610141) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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