GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric)
概述
产品名称
GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within Human GFAP aa 1-50 / 432.
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P, WB, mIHC
分子量
Predicted band size: 50 kDa
阳性对照
Human brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
SA03-04
反应性数据
| IHC-Fr | IHC-P | WB | mIHC | |
|---|---|---|---|---|
| Mouse |
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| Rat |
|
|
|
|
| Human |
|
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产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-Fr
-
1:1,000
-
IHC-P
-
1:500
-
WB
-
1:2,000
-
mIHC
-
1:1,000
靶点
功能
Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys, Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
背景文献
1. Zhang N et al. A self-assembly peptide nanofibrous scaffold reduces inflammatory response and promotes functional recovery in a mouse model of intracerebral hemorrhage. Nanomedicine N/A:N/A (2016).
2. Green AL et al. Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma. Neuro Oncol 17:697-707 (2015).
亚细胞定位
Cytoplasm
别名
wu:fb34h11 antibody
ALXDRD antibody
cb345 antibody
etID36982.3 antibody
FLJ42474 antibody
FLJ45472 antibody
GFAP antibody
GFAP_HUMAN antibody
gfapl antibody
Glial fibrillary acidic protein antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of GFAP on different lysates with Rat anti-GFAP antibody (HA601483) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601483) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Glioblastoma induces CAF-like astrocyte activation via the AKT/mTOR–SERPINH1/COL5A1 axis
期刊: Biomolecules And Biomedicine
DOI: 10.17305/bb.2025.11898
IF: 2.2
应用: IHC
反应种属: Mouse
发表时间: 2025 Jun
-
Gastrodin Attenuates Cerebral Ischemia–Reperfusion Injury by Enhancing Mitochondrial Fusion and Activating the AMPK‐OPA1 Signaling Pathway
期刊: CNS Neuroscience & Therapeutics
DOI: 10.1111/cns.70559
IF: 5
应用: WB
反应种属: Rat
发表时间: 2025 Aug
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