GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)
Catalog# HA601389
GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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HA610232
不含抗保成分
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Human
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein.
产品特异性
This Rat Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (HA722750). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
种属反应性
Mouse, Rat (Predicted: Human, Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-Fr, IHC-P, WB
分子量
Predicted band size: 65 kDa
阳性对照
Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH06-80
反应性数据
| IHC-Fr | IHC-P | WB | |
|---|---|---|---|
| Mouse |
|
|
|
| Rat |
|
|
|
| Human |
|
|
|
| Cynomolgus Monkey |
|
|
|
| Pig |
|
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:2,000
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IHC-P
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1:1,000
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WB
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1:2,000
靶点
功能
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
背景文献
1. Budhram A et al. Clinical spectrum of high-titre GAD65 antibodies. J Neurol Neurosurg Psychiatry. 2021 Feb
2. Budhram A et al. Positive Predictive Value of Anti-GAD65 ELISA Cut-Offs for Neurological Autoimmunity. Can J Neurol Sci. 2023 Sep
亚细胞定位
Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane.
别名
65 kDa glutamic acid decarboxylase antibody
DCE 2 antibody
DCE2 antibody
DCE2_HUMAN antibody
GAD 2 antibody
GAD 65 antibody
GAD-2 antibody
GAD-65 antibody
Gad2 antibody
Glutamate decarboxylase 2 (pancreas) antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of GAD65 on different lysates with Rat anti-GAD65 antibody (HA601389) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 60 kDa
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601389) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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