Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R]
Catalog# HA601301
Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R]
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WB
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Human
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Mouse
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Rat
概述
产品名称
Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant full length protein of Human PRDX6.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 25 kDa
阳性对照
HeLa cell lysate, K-562 cell lysate, mouse lung tissue lysate, mouse liver tissue lysate, rat lung tissue lysate, rat liver tissue lysate.
偶联
unconjugated
克隆号
7G1-R
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
靶点
功能
Peroxiredoxin-6 is a protein that in humans is encoded by the PRDX6 gene. It is a member of the peroxiredoxin family of antioxidant enzymes. Peroxiredoxin 6 is widely distributed in several organs, especially the lungs. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
背景文献
1. Kang S W et al. Characterization of a mammalian peroxiredoxin that contains one conserved cysteine. J Biol Chem 273:6303-6311 (1998).
2. Chen J-W et al. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities. J Biol Chem 275:28421-28427 (2000).
亚细胞定位
Cytoplasm. Lysosome.
别名
1 Cys antibody
1 Cys peroxiredoxin antibody
1 Cys PRX antibody
1 cysPrx antibody
1-Cys peroxiredoxin antibody
1-Cys PRX antibody
24 kDa protein antibody
9430088D19Rik antibody
AA690119 antibody
Acidic calcium independent phospholipase A2 antibody
展开图片
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Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA601301) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (40 µg/Lane)
Lane 5: Rat lung tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601301) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (HA601301) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Peroxiredoxin 6 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601301) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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Conjugate: unconjugated
