Peroxiredoxin 6 Mouse Monoclonal Antibody [7G1]
Catalog# EM1701-73
Peroxiredoxin 6 Mouse Monoclonal Antibody [7G1]
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WB
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IHC-P
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IF-Cell
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FC
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Human
概述
产品名称
Peroxiredoxin 6 Mouse Monoclonal Antibody [7G1]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant full length protein of Human PRDX6.
种属反应性
Human
验证应用
WB, IHC-P, IF-Cell, FC
分子量
Predicted band size: 25 kDa
阳性对照
PC-3M, K562, A431, SiHa, human liver tissue, human colon cancer tissue, human kidney tissue, human placenta tissue.
偶联
unconjugated
克隆号
7G1
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:100-1:400
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FC
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1:50-1:100
靶点
功能
Peroxiredoxin-6 is a protein that in humans is encoded by the PRDX6 gene. It is a member of the peroxiredoxin family of antioxidant enzymes. Peroxiredoxin 6 is widely distributed in several organs, especially the lungs. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
背景文献
1. Kang S W et al. Characterization of a mammalian peroxiredoxin that contains one conserved cysteine. J Biol Chem 273:6303-6311 (1998).
2. Chen J-W et al. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities. J Biol Chem 275:28421-28427 (2000).
序列相似性
Belongs to the peroxiredoxin family. Prx6 subfamily.
翻译后修饰
Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.; Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme.
亚细胞定位
Cytoplasm. Lysosome.
UNIPROT
别名
1 Cys antibody
1 Cys peroxiredoxin antibody
1 Cys PRX antibody
1 cysPrx antibody
1-Cys peroxiredoxin antibody
1-Cys PRX antibody
24 kDa protein antibody
9430088D19Rik antibody
AA690119 antibody
Acidic calcium independent phospholipase A2 antibody
展开图片
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Western blot analysis of PRDX6 on PC-3M (1) and K562 (2) using anti-PRDX6 antibody at 1/500 dilution.
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ICC staining PRDX6 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining PRDX6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining PRDX6 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PRDX6 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of PC-3M cells with PRDX6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
Peroxiredoxin 6 Recombinant Mouse Monoclonal Antibody [7G1-R] - BSA and Azide free
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Reactivity: Human,Mouse,Rat
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