LAMP2 Recombinant Mouse Monoclonal Antibody [D3-D8-D3-R]
Catalog# HA601192
LAMP2 Recombinant Mouse Monoclonal Antibody [D3-D8-D3-R]
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WB
-
IHC-P
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Human
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HA610072
不含抗保成分
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unconjugated
概述
产品名称
LAMP2 Recombinant Mouse Monoclonal Antibody [D3-D8-D3-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein corresponding to N terminal Human LAMP2.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 45 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HUVEC cell lysate, JAR cell lysate, HEK-293 cell lysate, THP-1 cell lysate, human kidney tissue, human liver tissue.
偶联
unconjugated
克隆号
D3-D8-D3-R
RRID
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
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IHC-P
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1:5,000
靶点
功能
Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live . Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation . Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy . Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes . Required for normal degradation of the contents of autophagosomes . Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits . Is not required for efficient MHCII-mediated presentation of endogenous antigens .
背景文献
1. Cuervo A.M.et.al.A receptor for the selective uptake and degradation of proteins by lysosomes.Science 273:501-503(1996).
亚细胞定位
Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane.
UNIPROT
别名
CD107 antigen like family member B antibody
CD107 antigen-like family member B antibody
CD107b antibody
LAMP 2 antibody
LAMP 2C antibody
LAMP-2 antibody
LAMP2 antibody
LAMP2_HUMAN antibody
LAMPB antibody
LGP 96 antibody
展开图片
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Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (HA601192) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HUVEC cell lysate
Lane 5: JAR cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 110 kDa
Exposure time: 3 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (HA601192) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-LAMP2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601192) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP2 antibody (HA601192) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601192) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-LAMP2 antibody (HA601192) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601192) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
LAMP2 Recombinant Mouse Monoclonal Antibody [D3-D8-D3-R] - BSA and Azide free
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Conjugate: unconjugated
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Conjugate: unconjugated
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