TMED5 Rabbit Polyclonal Antibody
Rmb: 1500
Catalog# HA500347
TMED5 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
TMED5 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within Human TMED5 aa 1-200 / 229.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
靶点分子量
Predicted band size: 26 kDa
阳性对照
HeLa cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse liver tissue lysate, rat bladder tissue, mouse large intestine tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:100,000
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IHC-P
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1:600-1:5,000
靶点
功能
Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Required for the maintenance of the Golgi apparatus; involved in protein exchange between Golgi stacks during assembly. Probably not required for COPI-vesicle-mediated retrograde transport. TMED5 is a part of the p24 protein family whose general functions are protein trafficking for the secretory pathway. TMED5 is thought to be necessary in the formation of the Golgi into a ribbon. Glycosylphosphatidylinositol-anchored proteins (GPI-AP) depend on p24 cargo receptors for transport from the ER to the Golgi. Knockdown of p24γ2 (a mouse ortholog of TMED5) in mice resulted in impaired transport of GPI-AP. The study concluded that the α-helical region of p24γ2 binds GPI which is necessary to incorporate it into COPII transport vesicles. TMED5 is reported to be necessary for the secretion of Wnt ligands. TMED5 has been found to interact with WNT7B, activating the canonical WNT-CTNNB1/β-catenin signaling pathway. This pathway is linked to numerous cancers because upregulation of the Wnt/β-catenin signaling pathway leads to cytosolic accumulation of β-catenin, promoting cellular proliferation.
背景文献
1. Bao C. et. al. TP73-AS1 promotes gastric cancer proliferation and invasion by regulation miR-27b-3p/TMED5 axis. J Cancer. 2022 Feb
2. Zou H. et. al. Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory network in cervical cancer development. World J Surg Oncol. 2021 Aug
亚细胞定位
Cis-Golgi network membrane, endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane.
别名
CGI 100 antibody
DKFZp781B2248 antibody
p24 family protein gamma-2 antibody
p24gamma2 antibody
p28 antibody
RP5 976O13.2 antibody
TMED 5 antibody
TMED5 antibody
TMED5_HUMAN antibody
Transmembrane emp24 domain containing protein 5 antibody
展开图片
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Western blot analysis of TMED5 on different lysates with Rabbit anti-TMED5 antibody (HA500347) at 1/100,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: RAW264.7 cell lysate (10 µg/Lane)
Lane 3: C6 cell lysate (10 µg/Lane)
Lane 4: Mouse liver tissue lysate (10 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500347) at 1/100,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-TMED5 antibody (HA500347) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500347) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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