iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
Rmb: 300
Catalog# HA1147
iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
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IHC-Fr
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IF-Tissue
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IF-Cell
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FC
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Guinea pig
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iFluor™ 647
概述
产品名称
iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
抗体类型
Goat Polyclonal Antibody
免疫原
Guinea pig IgG protein.
种属反应性
Guinea pig
验证应用
IHC-Fr, IF-Tissue, IF-Cell, FC
偶联
iFluor™ 647
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| IHC-Fr | FC | IF-Cell | IF-Tissue | |
|---|---|---|---|---|
| species independent |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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IF-Cell
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1:1,000
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FC
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1:200-1:500
靶点
功能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
背景文献
暂无
图片
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Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Brain
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601368, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601368, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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