iFluor™ 750 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody
Catalog# HA1134
iFluor™ 750 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody
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IF-Cell
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IF-Tissue
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FC
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Rabbit
概述
产品名称
iFluor™ 750 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody
抗体类型
Goat Polyclonal Antibody
免疫原
Rabbit IgG (H+L).
产品特异性
To minimize cross-reactivity, the iFluor™ 750 conjugated goat anti-rabbit IgG (H+L) antibodies have been highly cross-adsorbed against human IgG and Mouse IgG.
种属反应性
Rabbit
验证应用
IF-Cell, IF-Tissue, FC
偶联
iFluor™ 750
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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FC
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1:1,000
靶点
功能
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
背景文献
暂无
图片
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Flow cytometric analysis of HeLa cells labeling GAPDH.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1134) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"