Glucagon Mouse Monoclonal Antibody
Catalog# EM1701-34
Glucagon Mouse Monoclonal Antibody
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IHC
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IF-cell
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Human
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Mouse
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Rat
概述
产品名称
Glucagon Mouse Monoclonal Antibody
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant full length human glucagon protein.
种属反应性
Human, Mouse, Rat
验证应用
IHC, IF-cell
分子量
Predicted band size: 21 kDa
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
-15℃ to -25℃/1 year(Do not lower than -25℃)
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Immunogen affinity purified.
应用稀释度
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IHC
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1:200-1:400
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IF-cell
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1:50-1:200
靶点
功能
Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.
背景文献
1. Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017).
2. Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016).
序列相似性
Belongs to the glucagon family.
组织特异性
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
翻译后修饰
Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
亚细胞定位
Secreted.
别名
GCG antibody
图片
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点&同通路的产品
Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
