Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH21-00] - BSA and Azide free
Rmb: 5600
Catalog# HA751782
Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH21-00] - BSA and Azide free
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IHC-Fr
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IF-Cell
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FC
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Mouse
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HA724195
含抗保成分
-
unconjugated
概述
产品名称
Fas / CD95 Recombinant Rabbit Monoclonal Antibody [PSH21-00] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse Fas / CD95 aa 1-169.
种属反应性
Mouse
验证应用
IHC-Fr, IF-Cell, FC
靶点分子量
Predicted band size: 37 kDa
阳性对照
Mouse liver tissue, A20.
偶联
unconjugated
克隆号
PSH21-00
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
靶点
功能
The Fas receptor, also known as Fas, FasR, apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6), is a protein that in humans is encoded by the FAS gene. Fas was first identified using a monoclonal antibody generated by immunizing mice with the FS-7 cell line. Thus, the name Fas is derived from FS-7-associated surface antigen. The Fas receptor is a death receptor on the surface of cells that leads to programmed cell death (apoptosis) if it binds its ligand, Fas ligand (FasL). It is one of two apoptosis pathways, the other being the mitochondrial pathway.
背景文献
1. Galli G et al. Fas/CD95 Signaling Pathway in Damage-Associated Molecular Pattern (DAMP)-Sensing Receptors. Cells. 2022 Apr
2. Sica M et al. CD95/Fas stoichiometry in future precision medicine. Cell Death Differ. 2025 Sep
亚细胞定位
Cell membrane, Membrane raft.
UNIPROT
别名
ALPS 1A antibody
ALPS1A antibody
APO 1 antibody
Apo 1 antigen antibody
APO 1 cell surface antigen antibody
Apo-1 antigen antibody
APO1 antibody
Apo1 antigen antibody
APO1 cell surface antigen antibody
Apoptosis antigen 1 antibody
展开图片
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This data was developed using HA724195, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: liver
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
☑ Relative expression (RE)
This data was developed using HA724195, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A20 (positive) and L-929 (low expression) labeling Fas / CD95 with Rabbit anti-Fas / CD95 antibody (HA724195) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fas / CD95 antibody (HA724195) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
This data was developed using HA724195, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of L-929 (left, low expression) and A20 (right, positive) cells labeling Fas / CD95.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724195, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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