Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
Catalog# ET7110-10
Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
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WB
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IHC-P
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Human
概述
产品名称
Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB3.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 148 kDa
阳性对照
MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, human thyroid tissue, human skin tissue, human placenta tissue.
偶联
unconjugated
克隆号
JE51-89
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
靶点
功能
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.
背景文献
1. Sağsöz H. et. al. Expression and localisation of epidermal growth factor receptors and their ligands in the lower genital tract of cycling cows. Reprod Fertil Dev. 2019 Jul 4.
2. Dietrich M. et. al. Protein kinase C regulates ErbB3 turnover. Exp Cell Res. 2019 Jun 21.
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
组织特异性
Epithelial tissues and brain.
翻译后修饰
Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
亚细胞定位
Cell membrane, secreted.
UNIPROT #
别名
c erbB 3 antibody
c erbB3 antibody
Erb b2 receptor tyrosine kinase 3 antibody
ErbB 3 antibody
ERBB3 antibody
ERBB3 protein antibody
erbB3 S antibody
ERBB3_HUMAN antibody
Glial growth factor receptor antibody
HER 3 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-HER3/ErbB3 (Y1222) on different lysates with Rabbit anti-Phospho-HER3/ErbB3 (Y1222) antibody (ET7110-10) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate
Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 148 kDa
Observed band size: 180 kDa
Exposure time: 2 minutes 37 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"