Thyroglobulin Recombinant Rabbit Monoclonal Antibody [JE47-61]
Catalog# ET7109-64
Thyroglobulin Recombinant Rabbit Monoclonal Antibody [JE47-61]
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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Human
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unconjugated
概述
产品名称
Thyroglobulin Recombinant Rabbit Monoclonal Antibody [JE47-61]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Thyroglobulin aa 2,620-2,768 / 2,768.
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue, IF-Cell
分子量
Predicted band size: 305 kDa
阳性对照
Human thyroid gland tissue, human thyroid cancer tissue, TT.
偶联
unconjugated
克隆号
JE47-61
RRID
反应性数据
| WB | IHC-P | IF-Tissue | IF-Cell | |
|---|---|---|---|---|
| Human |
|
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500
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IHC-P
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1:1,000
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IF-Tissue
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1:200
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IF-Cell
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1:100
靶点
功能
Thyroglobulin (Tg) is a vertebrate secretory protein synthesized in the thyrocyte endoplasmic reticulum (ER), where it acquires N-linked glycosylation and conformational maturation (including formation of many disulfide bonds), leading to homodimerization. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Its primary functions include iodide storage and thyroid hormonogenesis. Tg consists largely of repeating domains, and many tyrosyl residues in these domains become iodinated to form monoiodo- and diiodotyrosine, whereas only a small portion of Tg structure is dedicated to hormone formation. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.
背景文献
1. Di Jeso B et al. Thyroglobulin From Molecular and Cellular Biology to Clinical Endocrinology. Endocr Rev 37(1):2-36 (2016).
序列相似性
Belongs to the type-B carboxylesterase/lipase family.
组织特异性
Specifically expressed in the thyroid gland.
翻译后修饰
Iodinated on tyrosine residues by TPO. There are 4 pairs of iodinated tyrosines used for coupling: acceptor Tyr-24 is coupled to donor Tyr-149 or Tyr-234, acceptor Tyr-2573 is coupled to donor Tyr-2540, acceptor Tyr-2766 in monomer 1 is coupled to donor Tyr-2766 in monomer 2 and acceptor Tyr-1310 in monomer 1 is coupled to donor Tyr-108 in monomer 2.; Sulfated tyrosines are desulfated during iodination.; Undergoes sequential proteolysis by cathepsins to release thyroxine (T4) and triiodothyronine (T3) hormones. In the thyroid follicle lumen, cross-linked TG (storage form) is solubilized by limited proteolysis mediated by cathepsins CTSB and/or CTSL. Partially cleaved TG is further processed by CTSK/cathepsin K and/or CTSL resulting in the release of T4. Following endocytosis, further processing occurs leading to the release of T3 and more T4 hormones.
亚细胞定位
Secreted.
UNIPROT
别名
AITD 3 antibody
AITD3 antibody
hTG antibody
TDH3 antibody
Tg antibody
TGN antibody
THYG_HUMAN antibody
Thyroglobulin antibody
图片
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Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Thyroglobulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-64, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-Thyroglobulin antibody (ET7109-64) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of TT cells labeling Thyroglobulin with Rabbit anti-Thyroglobulin antibody (ET7109-64) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Thyroglobulin antibody (ET7109-64) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Combining transfer of TTF-1 and Pax-8 gene: a potential strategy to promote radioiodine therapy of thyroid carcinoma
期刊: Cancer Gene Therapy
DOI: 10.1038/cgt.2012.13
IF: 2.802
应用: WB
反应种属: Human
发表时间: 2012 Apr
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