Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
Catalog# ET7106-82
Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
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WB
-
Human
-
Rat
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unconjugated
概述
产品名称
Coilin Recombinant Rabbit Monoclonal Antibody [JU44-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Coilin aa 1-50 / 576.
种属反应性
Human, Rat
验证应用
WB
分子量
Predicted band size: 63 kDa
阳性对照
HeLa cell lysate, 293T cell lysate, PC-12 cell lysate.
偶联
unconjugated
克隆号
JU44-08
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:5,000
靶点
功能
Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome and chromosome.
背景文献
1. Carmo-Fonseca M. al. Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis -- evidence that the coiled body is a kinetic nuclear structure. J. Cell Biol. 120:841-852(1993).
序列相似性
Belongs to the coilin family.
组织特异性
Found in all the cell types examined.
翻译后修饰
Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs.; Phosphorylation during mitosis is associated with disassembly of CBs.
亚细胞定位
Nucleus, Cajal body.
别名
CLN 80 antibody
CLN80 antibody
COIL antibody
COIL_HUMAN antibody
Coilin antibody
Coilin p80 antibody
p80 antibody
P80 coilin antibody
图片
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Western blot analysis of Coilin on different lysates with Rabbit anti-Coilin antibody (ET7106-82) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 75 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-82) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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