SMYD3 Recombinant Rabbit Monoclonal Antibody [JM73-63]
Catalog# ET1705-58
SMYD3 Recombinant Rabbit Monoclonal Antibody [JM73-63]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
SMYD3 Recombinant Rabbit Monoclonal Antibody [JM73-63]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SMYD3 aa 251-300 / 428.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
分子量
Predicted band size: 49 kDa
阳性对照
MCF7 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Hela, HepG2, LOVO, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue.
偶联
unconjugated
克隆号
JM73-63
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
靶点
功能
Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.
背景文献
1. Liu T et al. Master redox regulator Trx1 upregulates SMYD1 & modulates lysine methylation. Biochim Biophys Acta 1854:1816-22 (2015).
2. Wang HF et al. Associations of the variable number of tandem repeats polymorphism in the SMYD3 gene with risk and prognosis of esophageal cancer: a case-control study. Neoplasma. doi: 10.4149/neo_2017_613 (2017).
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.
组织特异性
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
亚细胞定位
Cytoplasm. Nucleus.
别名
bA74P14.1 (novel protein) antibody
bA74P14.1 antibody
FLJ21080 antibody
histone lysine N methyltransferase SMYD3 antibody
KMT3E antibody
MGC104324 antibody
SET and MYND domain containing 3 antibody
SET and MYND domain containing protein 3 antibody
SET and MYND domain-containing protein 3 antibody
SMYD 3 antibody
展开图片
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Western blot analysis of SMYD3 on different lysates with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate (10 µg/Lane)
Lane 2: HCT 116 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse spleen tissue lysate (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 49 kDa
Observed band size: 40 kDa
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-58) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of HepG2 cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of LOVO cells labeling SMYD3 with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-SMYD3 antibody (ET1705-58) at 1/50 dilution.Hela cells
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-58) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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