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NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26]

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Catalog# ET1703-75

NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human NMDAR1 aa 870-910.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Tissue, IHC-P, IHC-Fr

分子量

Predicted band size: 105 kDa

阳性对照

MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue.

偶联

unconjugated

克隆号

JM11-26

RRID

AB_3070430

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:200-1:500

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500

发表文章中的应用

WB See 1 publications below

发表文章中的种属

Rat See 1 publications below

靶点

功能

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+.Sensitivity to glutamate and channel kinetics depend on the subunit composition.

背景文献

1. Zhang X et al. A lasting effect of postnatal sevoflurane anesthesia on the composition of NMDA receptor subunits in rat prefrontal cortex. Int J Dev Neurosci 54:62-69 (2016).

2. Sloniecka M et al. Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ. PLoS One 10:e0134157 (2015).

序列相似性

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.

翻译后修饰

NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

亚细胞定位

Cell membrane, postsynaptic cell membrane, postsynaptic density.

UNIPROT #

SwissProt: Q05586 Human

SwissProt: P35438 Mouse

SwissProt: P35439 Rat

别名

GluN1 antibody

Glutamate [NMDA] receptor subunit zeta-1 antibody

Glutamate receptor ionotropic N methyl D aspartate 1 antibody

Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody

glutamate receptor ionotropic, NMDA 1 antibody

Grin1 antibody

MRD8 antibody

N methyl D aspartate receptor antibody

N methyl D aspartate receptor channel subunit zeta 1 antibody

N methyl D aspartate receptor subunit NR1 antibody

展开

图片

  • Immunofluorescence analysis of frozen mouse hippocampus tissue with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse hippocampus tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-75, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-75, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> <br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-75, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-75) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/5,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (15 µg/Lane)<br />Lane 2: Human brain tissue lysate (20 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br />Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane)<br />Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 105 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of NMDAR1 on different lysates with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/5,000 dilution.

    Lane 1: MCF7 cell lysate (15 µg/Lane)
    Lane 2: Human brain tissue lysate (20 µg/Lane)
    Lane 3: Mouse brain tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)
    Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane)
    Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 105 kDa
    Observed band size: 120 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-75) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NMDAR1 with Rabbit anti-NMDAR1 antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1703-75" style="font-weight: bold;text-decoration: underline;">ET1703-75</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.<br /><br />Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NMDAR1 with Rabbit anti-NMDAR1 antibody (ET1703-75) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-75, green) at 1/200 dilution overnight at 4 ℃, washed with PBS.

    Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  1. WB