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CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [JF10-09]

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Catalog# ET1702-92

CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [JF10-09]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [JF10-09]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human THY1 aa 75-120 / 161.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr

分子量

Predicted band size: 18 kDa

阳性对照

Hela cell lysate, HepG2 cell lysate, HUVEC cell lysate, human lung carcinoma tissue, human kidney tissue, human tonsil tissue, human spleen tissue, mouse brain tissue, mouse hippocampus tissue.

偶联

unconjugated

克隆号

JF10-09

RRID

AB_3070351

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:400-1:1,000

  • IHC-P

  • 1:2,000-1:4,000

  • IHC-Fr

  • 1:100-1:200

发表文章中的应用

IHC-P 查看 1 篇文献如下
IF 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a "cluster of differentiation" (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant.

背景文献

1. Chen WC et al. Cancer stem cell marker CD90 inhibits ovarian cancer formation via 3 integrin. Int J Oncol 49:1881-1889 (2016).

2. Du M et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017).

亚细胞定位

Cell membrane.

UNIPROT

SwissProt: P04216 Human

SwissProt: P01831 Mouse

SwissProt: P01830 Rat

别名

CD7 antibody

CD90 antibody

CD90 antigen antibody

CDw90 antibody

FLJ33325 antibody

MGC128895 antibody

T25 antibody

Theta antigen antibody

Thy 1 antibody

Thy 1 cell surface antigen antibody

展开

图片

  • Western blot analysis of CD90 / THY1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Hela cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: HUVEC cell lysate

    Western blot analysis of CD90 / THY1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HUVEC cell lysate

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />All lanes: Western blot analysis of THY1 with anti-CD90 / THY1 antibody[JF10-09] (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2: THY1 knockdown Hela whole cell lysate (10 µg).<br /><br /><a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a> was shown to specifically react with THY1 in wild-type Hela cells. Weakened bands were observed when THY1 knockdown samples were tested. Wild-type and THY1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    All lanes: Western blot analysis of THY1 with anti-CD90 / THY1 antibody[JF10-09] (ET1702-92) at 1/500 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2: THY1 knockdown Hela whole cell lysate (10 µg).

    ET1702-92 was shown to specifically react with THY1 in wild-type Hela cells. Weakened bands were observed when THY1 knockdown samples were tested. Wild-type and THY1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/4,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/4,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/100<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1/100

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/100<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/100

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Human<br /><br />Site: Kidney<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/400

    Application: IF-tissue

    Species: Human

    Site: Kidney

    Sample: Paraffin-embedded section

    Antibody concentration: 1/400

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/400

    Application: IF-tissue

    Species: Mouse

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1/400

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. IHC-P
  2. IF

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