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Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943]

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Catalog# ET1702-15

Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IHC-Fr

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

预测的种属支持售后
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.

种属反应性

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

预测的种属支持售后

验证应用

WB, IHC-P, IF-Tissue, IHC-Fr, mIHC

分子量

Predicted band size: 33 kDa

阳性对照

Rat brain tissue lysate, Mouse brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue.

偶联

unconjugated

克隆号

JF0943

RRID

AB_3070278

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000-1:30,000

  • IF-Tissue

  • 1:500-1:1,000

  • IHC-Fr

  • 1:500-1:1,000

  • mIHC

  • 1:2,000

发表文章中的应用

WB 查看 2 篇文献如下
IHC-P 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下
Rat 查看 1 篇文献如下

靶点

功能

Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.

背景文献

1. Martinsen V et al. Multiple sclerosis and myelin basic protein: insights into protein disorder and disease. Amino Acids. 2022 Jan

2. Bagheri H et al. Myelin basic protein mRNA levels affect myelin sheath dimensions, architecture, plasticity, and density of resident glial cells. Glia. 2024 Oct

序列相似性

Belongs to the myelin basic protein family.

组织特异性

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

翻译后修饰

Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.; The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).; Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.; Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

亚细胞定位

Myelin membrane, Nucleus.

UNIPROT

SwissProt: P02686 Human

SwissProt: P04370 Mouse

SwissProt: P02688 Rat

别名

GDB antibody

Golli MBP antibody

Golli MBP

myelin basic protein antibody

Hemopoietic MBP antibody

HMBPR antibody

HUGO antibody

MBP antibody

MBP_CAVPO antibody

MBP_HUMAN antibody

展开

图片

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:1,000

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Human<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:500

    Application: IF-tissue

    Species: Human

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1:500

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:500

    Application: IF-tissue

    Species: Mouse

    Site: Hippocampus

    Sample: Paraffin-embedded section

    Antibody concentration: 1:500

  • Western blot analysis of Myelin Basic Protein on different lysates with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/500 dilution.<br /><br />Lane 1: Rat brain tissue lysate<br />Lane 2: Mouse brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 14~25 kDa<br /><br />Exposure time: 2 minutes;<br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myelin Basic Protein on different lysates with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/500 dilution.

    Lane 1: Rat brain tissue lysate
    Lane 2: Mouse brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 14~25 kDa

    Exposure time: 2 minutes;
    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-15) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-15) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/30,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-15) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Myelin Basic Protein antibody (<a href="/products/ET1702-15" style="font-weight: bold;text-decoration: underline;">ET1702-15</a>) at 1/2,000 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Myelin Basic Protein antibody (ET1702-15) at 1/2,000 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P

同靶点 & 同通路的产品

metafield image

Myelin Basic Protein Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Tissue,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943] - BSA and Azide free

Application: WB,IHC-P,IF-Tissue,IHC-Fr

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

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