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KAP1 Recombinant Rabbit Monoclonal Antibody [SD081-05]

RMB: 900 特惠 1500

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Catalog# ET1612-55

KAP1 Recombinant Rabbit Monoclonal Antibody [SD081-05]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

KAP1 Recombinant Rabbit Monoclonal Antibody [SD081-05]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human KAP1 aa 301-350 / 835.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Tissue, IHC-P

分子量

Predicted band size: 89 kDa

阳性对照

F9 cell lysate, C6 cell lysate, Human lung tissue lysate, Mouse colon tissue lysate, human kidney tissue, mouse brain tissue, rat kidney tissue.

偶联

unconjugated

克隆号

SD081-05

RRID

AB_3070129

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:3,000

靶点

功能

TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein.

背景文献

1. Thompson PJ et al. hnRNP K Coordinates Transcriptional Silencing by SETDB1 in Embryonic Stem Cells. PLoS Genet 11:e1004933 (2015).

2. Gjyshi O et al. Activated Nrf2 Interacts with Kaposi\'s Sarcoma-Associated Herpesvirus Latency Protein LANA-1 and Host Protein KAP1 To Mediate Global Lytic Gene Repression. J Virol 89:7874-92 (2015).

序列相似性

Belongs to the TRIM/RBCC family.

组织特异性

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

翻译后修饰

ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.; Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.; Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2.; Citrullinated by PADI4.

亚细胞定位

Nucleus.

UNIPROT

SwissProt: Q13263 Human

SwissProt: Q62318 Mouse

SwissProt: O08629 Rat

别名

E3 SUMO protein ligase TRIM28 antibody

E3 SUMO-protein ligase TRIM28 antibody

FLJ29029 antibody

KAP 1 antibody

KAP-1 antibody

KRAB associated protein 1 antibody

KRAB interacting protein 1 antibody

KRAB-associated protein 1 antibody

KRAB-interacting protein 1 antibody

KRIP 1 antibody

展开

图片

  • Western blot analysis of KAP1 on different lysates with Rabbit anti-KAP1 antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/2,000 dilution.<br /><br />Lane 1: F9 cell lysate (20 µg/Lane)<br />Lane 2: C6 cell lysate (20 µg/Lane)<br />Lane 3: Human lung tissue lysate (40 µg/Lane)<br />Lane 4: Mouse colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 89 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KAP1 on different lysates with Rabbit anti-KAP1 antibody (ET1612-55) at 1/2,000 dilution.

    Lane 1: F9 cell lysate (20 µg/Lane)
    Lane 2: C6 cell lysate (20 µg/Lane)
    Lane 3: Human lung tissue lysate (40 µg/Lane)
    Lane 4: Mouse colon tissue lysate (40 µg/Lane)

    Predicted band size: 89 kDa
    Observed band size: 110 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-55) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KAP1 antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KAP1 antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-KAP1 antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-55" style="font-weight: bold;text-decoration: underline;">ET1612-55</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-KAP1 antibody (ET1612-55) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-55) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IF
  3. IHC-P

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