SOX10 Recombinant Rabbit Monoclonal Antibody [SN20-41]
Catalog# ET1611-89
SOX10 Recombinant Rabbit Monoclonal Antibody [SN20-41]
-
WB
-
Human
-
Mouse
-
Rat
预测的种属支持售后
-
unconjugated
概述
产品名称
SOX10 Recombinant Rabbit Monoclonal Antibody [SN20-41]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SOX10 aa 400-450 / 466.
种属反应性
Human, Mouse (Predicted: Rat)
预测的种属支持售后
验证应用
WB
分子量
Predicted band size: 50 kDa
阳性对照
SK-MEL-28 cell lysate, B16-F1 cell lysate, A375 cell lysate.
偶联
unconjugated
克隆号
SN20-41
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
靶点
功能
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
背景文献
1. Guye P et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016).
2. Arts N et al. microRNA-155, induced by interleukin-1 , represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS One 10:e0122517 (2015).
组织特异性
Expressed in fetal brain and in adult brain, heart, small intestine and colon.
亚细胞定位
Cytoplasm, Nucleus, Mitochondrion outer membrane.
别名
DOM antibody
DOM antibody
Dominant megacolon mouse human homolog of antibody
MGC15649 antibody
PCWH antibody
SOX 10 antibody
SOX10 antibody
SOX10_HUMAN antibody
SRY (sex determining region Y) box 10 antibody
SRY (sex determining region Y) box 10 antibody
展开图片
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Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (ET1611-89) at 1/5,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: B16-F1 cell lysate
Lane 3: A375 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55/75 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-89) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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