NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
概述
产品名称
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human NSE aa 30-70.
种属反应性
Human, Mouse, Rat (Predicted: Zebrafish)
预测的种属支持售后
验证应用
WB
分子量
Predicted band size: 47 kDa
阳性对照
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, PC-12 cell lysate, SH-SY5Y cell lysate, hybrid fish (crucian-carp) brain tissue lysate, human breast cancer tissue , SH-SY5Y, 293, rat brain tissue, mouse brain tissue.
偶联
unconjugated
克隆号
SC06-28
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:5,000
靶点
功能
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
背景文献
1. Chang BS et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015).
2. Sun Y et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014).
序列相似性
Belongs to the enolase family.
组织特异性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
亚细胞定位
Cytoplasm, Cell membrane.
别名
2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma, neuronal) antibody
Enolase 2 antibody
Enolase 2 gamma neuronal antibody
Enolase2 antibody
展开图片
-
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: HEK-293 cell lysate (10 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: SH-SY5Y cell lysate (10 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NSE KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NSE on hybrid fish
(crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Characteristics of endoplasmic reticulum stress changes during the differentiation of adipose-derived stromal cells into neurons
期刊: Cytotechnology
DOI: 10.1007/s10616-025-00891-8
IF: 1.7
应用: IHC,WB
反应种属: Human
发表时间: 2026 Jan
-
BRAF Mutation as a Potential Therapeutic Target for Checkpoint Inhibitors: A Comprehensive Analysis of Immune Microenvironment in BRAF Mutated Colon Cancer
期刊: Frontiers In Cell And Developmental Biology
DOI:
IF: 6.68
应用: IHC-P
反应种属: Human
发表时间: 2021 Jul
同靶点 & 同通路的产品
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 488
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
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