iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Catalog# HA720178F
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
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IF-Cell
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FC
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Human
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Mouse
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Rat
概述
产品名称
iFluor™ 488 Conjugated NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human NSE aa 30-70.
种属反应性
Human, Mouse, Rat
验证应用
IF-Cell, FC
分子量
Predicted band size: 47 kDa
阳性对照
SH-SY5Y.
偶联
iFluor™ 488
克隆号
SC06-28
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Cell
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1:50
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FC
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1:500-1:1,000
靶点
功能
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
背景文献
1. Chang BS et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015).
2. Sun Y et al. The expression and significance of neuronal iconic proteins in podocytes. PLoS One 9:e93999 (2014).
序列相似性
Belongs to the enolase family.
组织特异性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
亚细胞定位
Cytoplasm, Cell membrane.
别名
2 phospho D glycerate hydrolyase antibody
2-phospho-D-glycerate hydro-lyase antibody
Eno 2 antibody
ENO2 antibody
ENOG antibody
ENOG_HUMAN antibody
Enolase 2 (gamma, neuronal) antibody
Enolase 2 antibody
Enolase 2 gamma neuronal antibody
Enolase2 antibody
展开图片
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Immunocytochemistry analysis of SH-SY5Y cells labeling NSE with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of SH-SY5Y cells labeling NSE.
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with NSE (HA720178F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
NSE Recombinant Rabbit Monoclonal Antibody [SC06-28]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
