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CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

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Catalog# ET1606-50

CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

Application

  • WB

  • IF-Cell

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human CD86 aa 1-50 / 329.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IP

分子量

Predicted band size: 38 kDa

阳性对照

Daudi cell lysate, Raji cell lysate, Ramos cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, Raji.

偶联

unconjugated

克隆号

SJ20-00

RRID

AB_3069745

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000-1:20,000

  • IF-Cell

  • 1:500

  • IP

  • Use at an assay dependent concentration.

靶点

功能

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells. Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. Roles of both CD80 and CD86 are studied in context of many pathologies. Selective inhibition of costimulatory inhibitors was examined in a model of allergic pulmonary inflammation and airway hyper-responsiveness (AHR). Since initial host response to Staphylococcus aureus, especially the immune response based on T cells, is a contributing factor in the pathogenesis of acute pneumonia, role of the CD80/CD86 pathway in pathogenesis was investigated. The costimulatory molecules were also investigated in context of Bronchial Astma, Treg in cancer, and immunotherapy.

背景文献

1. Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015).

2. Tarhini AA et al. Immune monitoring of the circulation and the tumor microenvironment in patients with regionally advanced melanoma receiving neoadjuvant ipilimumab. PLoS One 9:e87705 (2014).

组织特异性

Expressed by activated B-lymphocytes and monocytes.

翻译后修饰

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

亚细胞定位

Cell membrane.

UNIPROT

SwissProt: P42081 Human

SwissProt: P42082 Mouse

Entrez Gene: 56822 Rat

别名

Activation B7-2 antigen 3 antibody

Activation B7-2 antigen antibody

B-lymphocyte activation antigen B7-2 2 antibody

B-lymphocyte activation antigen B7-2 antibody

B7 2 antibody

B7 antibody

B7-2 antibody

B7.2 antibody

B70 antibody

B72 antigen antibody

展开

图片

  • Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/5,000 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: Ramos cell lysate<br />Lane 4: K-562 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 2 minutes 37 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Ramos cell lysate
    Lane 4: K-562 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 70 kDa

    Exposure time: 2 minutes 37 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/5,000 dilution.<br /><br />Lane 1: Raji cell lysate (10 µg/Lane)<br />Lane 2: Mouse lung tissue lysate (20 µg/Lane)<br />Lane 3: Rat lung tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution.

    Lane 1: Raji cell lysate (10 µg/Lane)
    Lane 2: Mouse lung tissue lysate (20 µg/Lane)
    Lane 3: Rat lung tissue lysate (20 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 70 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/500 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody (<a href="/products/ET1606-50" style="font-weight: bold;text-decoration: underline;">ET1606-50</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IF
  3. IHC

同靶点 & 同通路的产品

metafield image

CD86 Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Tissue,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

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