Skip to content

Pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SZ02-08]

RMB: 1500

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# ET1603-26

Pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SZ02-08]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Monkey

Predicted reactivity

  • Mouse

预测的种属支持售后
This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SZ02-08]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human pro Caspase-3 aa 18-67 / 277.

种属反应性

Human, Monkey (Predicted: Mouse)

预测的种属支持售后

验证应用

WB, IF-Tissue, IHC-P

分子量

Predicted band size: 32 kDa

阳性对照

HCT 116 cell lysate, Jurkat cell lysate, COS-1 cell lysates, human tonsil tissue.

偶联

unconjugated

克隆号

SZ02-08

RRID

AB_3069676

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

发表文章中的应用

WB 查看 1 篇文献如下
IHC 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下
Human 查看 1 篇文献如下

靶点

功能

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.

背景文献

1. Abdi J et al. Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells. Blood Cancer J 3:e119 (2013).

2. Nilsonne G et al. Phenotype-dependent apoptosis signalling in mesothelioma cells after selenite exposure. J Exp Clin Cancer Res 28:92 (2009).

序列相似性

Belongs to the peptidase C14A family.

组织特异性

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

翻译后修饰

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

亚细胞定位

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P42574 Human

SwissProt: P70677 Mouse

别名

Caspase3

A830040C14Rik antibody

Apopain antibody

CASP 3 antibody

CASP-3 antibody

CASP3 antibody

CASP3_HUMAN antibody

Casp3a antibody

Caspase 3 antibody

Caspase 3, apoptosis-related cysteine peptidase antibody

展开

图片

  • Western blot analysis of Pro Caspase-3 on different lysates with Rabbit anti-Pro Caspase-3 antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate<br />Lane 2: Jurkat cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Pro Caspase-3 on different lysates with Rabbit anti-Pro Caspase-3 antibody (ET1603-26) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate
    Lane 2: Jurkat cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Pro Caspase-3 on COS-1 cell lysates with Rabbit anti-Pro Caspase-3 antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 32 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Pro Caspase-3 on COS-1 cell lysates with Rabbit anti-Pro Caspase-3 antibody (ET1603-26) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-26) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Pro Caspase-3 on different lysates with Rabbit anti-Pro Caspase-3 antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Pro Caspase-3 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 180 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Pro Caspase-3 on different lysates with Rabbit anti-Pro Caspase-3 antibody (ET1603-26) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Pro Caspase-3 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 180 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-26) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Pro Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1603-26" style="font-weight: bold;text-decoration: underline;">ET1603-26</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Pro Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC

同靶点 & 同通路的产品

metafield image

Pro Caspase-3 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC,IHC-Fr

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

Pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR03-01]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Reactivity: Human,Monkey

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

请您留言

杭州华安生物技术有限公司 0571-88062880

您需要的服务
抗体咨询与购买
提交