CH25H Rabbit Polyclonal Antibody
Rmb: 1500
Catalog# ER1919-29
CH25H Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
CH25H Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human CH25H aa 51-100.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
靶点分子量
Predicted band size: 32 kDa
阳性对照
Vero (African green monkey normal kidney cell) cell lysate, U-87 MG (Human glioblastoma cell) cell lysate, MG-63 (Human osteosarcoma cell) cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate, Rat kidney tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
反应性数据
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
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||
| Mouse |
|
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| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:10,000
靶点
功能
CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
背景文献
1. Lu Z et al. ATF3 and CH25H regulate effector trogocytosis and anti-tumor activities of endogenous and immunotherapeutic cytotoxic T lymphocytes. Cell Metab. 2022 Sep
2. Zhang Y et al. Novel CH25H(+) and OASL(+) microglia subclusters play distinct roles in cerebral ischemic stroke. J Neuroinflammation. 2023 May
亚细胞定位
Endoplasmic reticulum membrane.
别名
C25H antibody
Ch25h antibody
CH25H_HUMAN antibody
Cholesterol 25 hydroxylase antibody
Cholesterol 25 monooxygenase antibody
Cholesterol 25-hydroxylase antibody
Cholesterol 25-monooxygenase antibody
EC 1.14.99.38 antibody
EC=1.14.99.38 antibody
h25OH antibody
图片
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Western blot analysis of CH25H on different lysates with Rabbit anti-CH25H antibody (ER1919-29) at 1/10,000 dilution.
Lane 1: Vero (African green monkey normal kidney cell) cell lysate
Lane 2: U-87 MG (Human glioblastoma cell) cell lysate
Lane 3: MG-63 (Human osteosarcoma cell) cell lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Rat kidney tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 1minute 50 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1919-29, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 32 kDa
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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