GFM2 Rabbit Polyclonal Antibody
Catalog# ER1909-51
GFM2 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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IF-Tissue
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Human
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Rat
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unconjugated
概述
产品名称
GFM2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human GFM2 151-250/779
种属反应性
Human, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
87 KDa
阳性对照
Widely expressed.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
-
1:100-500
靶点
功能
Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.
背景文献
暂无
亚细胞定位
Mitochondrial.
UNIPROT
别名
EF-G2mt antibody
EFG2 antibody
EFG2mt antibody
elongation factor G 2
mitochondrial antibody
Elongation factor G2 antibody
G elongation factor mitochondrial 2 antibody
GFM2 antibody
hEFG2 antibody
mEFG 2 antibody
展开图片
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Protein: A549(human) lyates at 40ug;
Primary: Rabbit Anti-GFM2 (ER1909-51) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:87 kD
Observed band size:63 kD -
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFM2) Polyclonal Antibody, Unconjugated (ER1909-51) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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