NOX2 / gp91phox Rabbit Polyclonal Antibody
Catalog# ER1511-34
NOX2 / gp91phox Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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FC
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WB
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Human
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Mouse
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unconjugated
概述
产品名称
NOX2 / gp91phox Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide corresponding to C-terminal human NOX2.
种属反应性
Human, Mouse
验证应用
IF-Cell, IHC-P, FC, WB
分子量
Predicted band size: 65 kDa
阳性对照
RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, RAW264.7, human liver tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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IF-Cell
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1:50-1:200
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IHC-P
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1:500-1:1,000
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FC
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1:50-1:100
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WB
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1:2,000
靶点
功能
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.
背景文献
暂无
组织特异性
Detected in neutrophils (at protein level).
翻译后修饰
Glycosylated.; Phosphorylated on Ser and Thr residues.; Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation.
亚细胞定位
Cell membrane.
别名
AMCBX2 antibody
CGD antibody
CGD91-phox antibody
CY24B_HUMAN antibody
CYBB antibody
Cytochrome b 245, beta polypeptide antibody
Cytochrome b(558) beta chain antibody
Cytochrome b(558) subunit beta antibody
Cytochrome b-245 heavy chain antibody
Cytochrome b558 subunit beta antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: Mouse spleen tissue lysate (40 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of RAW264.7 cells labeling NOX2 / gp91phox with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NOX2 / gp91phox antibody (ER1511-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
NOX2 / gp91phox Recombinant Rabbit Monoclonal Antibody [PSH10-59]
Application: WB,IF-Cell,IHC-P,IHC-Fr,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NOX2 / gp91phox Recombinant Rabbit Monoclonal Antibody [PSH10-59] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,IHC-Fr,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NOX2 / gp91phox Recombinant Rabbit Monoclonal Antibody [SN07-16] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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