Caveolin-1 Mouse Monoclonal Antibody [19-D6]
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Catalog# EM40728
Caveolin-1 Mouse Monoclonal Antibody [19-D6]
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
Caveolin-1 Mouse Monoclonal Antibody [19-D6]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Mouse Caveolin-1 aa 129-178 / 178.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 20 kDa
阳性对照
A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat lung tissue lysate, mouse bladder tissue, rat lung tissue, human lung tissue, mouse lung tissue.
偶联
unconjugated
克隆号
19-D6
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500
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IHC-P
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1:200-1:1,000
靶点
功能
Caveolin, an integral membrane protein, is a principal component of caveolae membranes in vivo. Two isoforms of caveolin have been identified: a slower migrating 24-kDa species (-isoform) and a faster migrating 21-kDa species (-isoform). Caveolins interact with multiple signaling molecules, such as the G-protein alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS. Caveolin-1 has been implicated in the pathogenesis of mammary epithelial cell hyperplasia.
背景文献
1. Kaarteenaho R, et al. Epithelial N-cadherin and nuclear β-catenin are up-regulated during early development of human lung. BMC Dev Biol. 10:113 (2010)
2. Qayyum T, et al. The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder. Br J Cancer. 106:1187-95 (2012).
序列相似性
Belongs to the caveolin family.
组织特异性
Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.
翻译后修饰
Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation.; The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.; Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.
亚细胞定位
Cell membrane, Golgi apparatus membrane.
别名
BSCL3 antibody
CAV antibody
CAV1 antibody
CAV1_HUMAN antibody
caveolae protein, 22 kD antibody
caveolin 1 alpha isoform antibody
caveolin 1 beta isoform antibody
Caveolin 1 caveolae protein 22kDa antibody
Caveolin-1 antibody
Caveolin1 antibody
展开图片
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Western blot analysis of Caveolin-1 on different lysates with Mouse anti-Caveolin-1 antibody (EM40728) at 1/500 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-Caveolin-1 antibody (EM40728) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40728) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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