iFluor™ 488 Conjugated E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287]

Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

Flow cytometric analysis of A431 cells labeling E-Cadherin.

Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Flow cytometric analysis of MCF-7 cells labeling E-Cadherin.

Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).