CFD Recombinant Rabbit Monoclonal Antibody [JE65-08]
Rmb: 699 特惠 1500
Catalog# HA722364
CFD Recombinant Rabbit Monoclonal Antibody [JE65-08]
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Human
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unconjugated
概述
产品名称
CFD Recombinant Rabbit Monoclonal Antibody [JE65-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human CFD aa 54-253 / 253.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, IF-Tissue
靶点分子量
Predicted band size: 27 kDa
阳性对照
THP-1 cell lysate, U-937 cell lysate, Human plasma lysate, Human serum lysate, A549 cell lysate, THP-1, human kidney tissue.
偶联
unconjugated
克隆号
JE65-08
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | FC | IF-Cell | IF-Tissue | |
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| human |
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| mouse |
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| rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IHC-P
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1:1,000--1:5,000
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IF-Tissue
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1:200-1:1,000
靶点
功能
Factor D (EC 3.4.21.46, C3 proactivator convertase, properdin factor D esterase, factor D (complement), complement factor D, CFD, adipsin) is a protein which in humans is encoded by the CFD gene. Factor D is involved in the alternative complement pathway of the complement system where it cleaves factor B. The protein encoded by this gene is a member of the trypsin family of serine proteases secreted by adipocytes into the bloodstream. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
背景文献
1. Gavriilaki E et al. Novel Insights into Factor D Inhibition. Int J Mol Sci. 2022 Jun
2. Risitano AM et al. Danicopan: an oral complement factor D inhibitor for paroxysmal nocturnal hemoglobinuria. Haematologica. 2021 Dec
亚细胞定位
Secreted.
UNIPROT
别名
Adipsin antibody
Adipsin/complement factor D antibody
Adn antibody
C3 convertase activator antibody
CFAD_HUMAN antibody
CFD antibody
Complement factor D antibody
complement factor D preproprotein antibody
D component of complement antibody
DF antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of CFD on different lysates with Rabbit anti-CFD antibody (HA722364) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: U-937 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (negative) (20 µg/Lane)
Lane 4: Human plasma lysate (40 µg/Lane)
Lane 5: Human serum lysate (40 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 25 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722364) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CFD on different lysates with Rabbit anti-CFD antibody (HA722364) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: Human serum lysate (40 µg/Lane)
Lane 5: Human plasma lysate (40 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 25 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722364) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of THP-1 cells labeling CFD with Rabbit anti-CFD antibody (HA722364) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CFD antibody (HA722364) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CFD antibody (HA722364) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722364) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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