CD4 Recombinant Antibody [ST0488] - Rat IgG1 (Chimeric) - BSA and Azide free

This data was developed using HA601671, the same antibody clone in a different buffer formulation.
Western blot analysis of CD4 on different lysates with Rat anti-CD4 antibody (HA601671) at 1/5,000 dilution.

Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 2: U-937 (Human acute monocytic leukemia cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute; ECL: K1803

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601671, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 51 kDa
Observed band size: 55 kDa

This data was developed using HA601671, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601671, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature.

This data was developed using HA601671, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Lymph nodes
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601671, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature.