CD276 Rabbit Polyclonal Antibody
Catalog# ER1803-36
CD276 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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Human
概述
产品名称
CD276 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human CD276 aa 1-466 / 534.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 57 kDa
阳性对照
MCF7 cell lysate, HEK-293 cell lysate, LoVo cell lysate, U-2 OS cell lysate, LNCaP cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, HCT 116 cell lysate, MCF7.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:2,000-1:5,000
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IF-Cell
-
1:500
靶点
功能
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
背景文献
1. Chapoval A.I et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2:269-274 (2001).
2. Steinberger P et al. Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains. J Immunol 172:2352-2359 (2004).
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
组织特异性
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
亚细胞定位
Membrane.
UNIPROT
别名
4Ig B7 H3 antibody
4Ig-B7-H3 antibody
AU016588 antibody
B7 H3 antibody
B7 homolog 3 antibody
B7-H3 antibody
B7H3 antibody
B7RP-2 antibody
CD_antigen=CD276 antibody
CD276 antibody
展开图片
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Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (ER1803-36) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: LoVo cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: LNCaP cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: THP-1 cell lysate
Lane 8: HCT 116 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 100 kDa
Exposure time: 42 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-36) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (ER1803-36) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD276 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 100 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-36) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF7 cells labeling CD276 with Rabbit anti-CD276 antibody (ER1803-36) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD276 antibody (ER1803-36) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
