CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63]
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Catalog# HA721321
CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
概述
产品名称
CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD14 aa 300-375.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 40 kDa
阳性对照
NIH/3T3 cell lysate, SiHa cell lysate, human colon carcinoma tissue, human liver tissue, human tonsil tissue.
偶联
unconjugated
克隆号
PD01-63
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:200
靶点
功能
CD14 is a 55-kDa protein found as a glycosylphosphatidylinositol (GPI)- anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, and as a soluble protein in the blood. Its main function is to serve as a receptor for lipopolysaccharide (LPS). CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14. Besides its role in endotoxin signaling, it has been proposed that CD14 is involved in the transportation of other lipids, cell-cell interactions during different immune responses, and recognition of apoptotic cells. CD14 is highly expressed on the surface of monocytes/macrophages and strongly up-regulated during the differentiation of monocytic precursor cells into mature monocytes. Therefore, CD14 has been commonly used as a differentiation marker for monocytes/macrophages. An antibody to CD14 also labels Langerhans' cells and dendritic cells.
背景文献
1. Ciesielska A et al. TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell Mol Life Sci. 2021 Feb
2. Nakamizo S et al. Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis. Julia V, Hacini-Rachinel F, Kabashima K, Ginhoux F.
3. J Exp Med. 2021 Sep
组织特异性
Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
翻译后修饰
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
亚细胞定位
Cell membrane, Secreted, Golgi apparatus, Membrane raft.
别名
CD 14 antibody
CD_antigen=CD14 antibody
CD14 antibody
CD14 antigen antibody
CD14 molecule antibody
CD14_HUMAN antibody
LPS-R antibody
Mo2 antibody
Monocyte differentiation antigen CD14 antibody
Monocyte differentiation antigen CD14 urinary form antibody
展开图片
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Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: SiHa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721321) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.
Lane 1: SW480-si NT cell lysate
Lane 2: SW480-si CD14 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 50-60 kDa
Exposure time: 25 seconds;
ECL: merk
4-20% SDS-PAGE gel.
HA721321 was shown to specifically react with CD14 in SW480-si NT cells. Weakened band was observed when SW480-si CD14 sample was tested. SW480-si NT and SW480-si CD14 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721321, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD14 antibody (HA721321) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: colon cancer
Sample: Paraffin-embedded section
Antibody concentration: 1/200
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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