AU5 tag Rabbit Polyclonal Antibody
Catalog# R1402-4
AU5 tag Rabbit Polyclonal Antibody
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WB
-
ELISA
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Species independent
概述
产品名称
AU5 tag Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein fused with AU5 Tag.
种属反应性
Species independent
验证应用
WB, ELISA
阳性对照
AU5 tagged fusion protein
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:5,000
-
ELISA
-
1:10,000
靶点
功能
AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.
背景文献
暂无
别名
AU5 epitope tag antibody
TDFYLK epitope tag antibody
TDFYLK tag antibody
图片
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Western blot analysis of AU5 tag on AU5 tagged fusion protein with Rabbit anti-AU5 tag antibody (R1402-4) at 1/1,000 dilution.
Lane 1: AU5 tagged fusion protein, 1,000ng/Lane
Lane 2: AU5 tagged fusion protein, 200ng/Lane
Lane 3: AU5 tagged fusion protein, 50ng/Lane
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"