AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09]
概述
产品名称
AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human AKT3 aa 300-479.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, IHC-Fr
分子量
Predicted band size: 56 kDa
阳性对照
MCF7 cell lysate, U-2 OS cell lysate, Jurkat cell lysate, C6 cell lysate, mouse heart tissue lysate, mouse testis tissue lysate, rat heart tissue lysate, rat testis tissue lysate, MCF7, RAW264.7, C6, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue, rat brain tissue, rat lung tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.
偶联
unconjugated
克隆号
ST48-09
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000-1:10,000
-
IF-Cell
-
1:100-1:200
-
IF-Tissue
-
1:500-1:1,000
-
IHC-P
-
1:2,000
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
-
IHC-Fr
-
1:100
靶点
功能
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
背景文献
1. Buendia, I. et al. 2015. The Melatonin-N,N-Dibenzyl(N-methyl)amine Hybrid ITH91/IQM157 Affords Neuroprotection in an in Vitro Alzheimer\'s Model via Hemo-oxygenase-1 Induction. ACS chemical neuroscience. 6: 288-96.
2. Siendones, E. et al. 2014. Membrane-bound CYB5R3 is a common effector of nutritional and oxidative stress response through FOXO3a and Nrf2. Antioxidants & redox signaling. 21: 1708-25.
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
组织特异性
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
翻译后修饰
O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.; Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Phosphorylated at Thr-308 and Ser-473 by IKBKE and TBK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells. Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP. Dephosphorylated at Thr-308 and Ser-473 by PP2A phosphatase. The phosphorylated form of PPP2R5B is required for bridging AKT1 with PP2A phosphatase. Ser-473 is dephosphorylated by CPPED1, leading to termination of signaling.; Ubiquitinated via 'Lys-48'-linked polyubiquitination by ZNRF1, leading to its degradation by the proteasome (By similarity). Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. Also ubiquitinated by TRIM13 leading to its proteasomal degradation. Phosphorylated, undergoes 'Lys-48'-linked polyubiquitination preferentially at Lys-284 catalyzed by MUL1, leading to its proteasomal degradation.; Acetylated on Lys-14 and Lys-20 by the histone acetyltransferases EP300 and KAT2B. Acetylation results in reduced phosphorylation and inhibition of activity. Deacetylated at Lys-14 and Lys-20 by SIRT1. SIRT1-mediated deacetylation relieves the inhibition.
亚细胞定位
Cell membrane, Cytoplasm, Membrane, Nucleus.
UNIPROT
别名
AKT antibody
AKT1 antibody
AKT1 kinase antibody
AKT1m antibody
AKT2 antibody
AKT2 kinase antibody
Akt3 antibody
AKT3_HUMAN antibody
CAKT antibody
CWS6 antibody
展开图片
-
Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: RAW264.7 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (20 µg/Lane)
Lane 10: Mouse heart tissue lysate (20 µg/Lane)
Lane 11: Mouse testis tissue lysate (20 µg/Lane)
Lane 12: Rat brain tissue lysate (20 µg/Lane)
Lane 13: Rat heart tissue lysate (20 µg/Lane)
Lane 14: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-51) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
AKT1/2/3 was immunoprecipitated from 0.2 mg MCF7 cell lysate with ET1609-51 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1609-51 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (input)
Lane 2: ET1609-51 IP in MCF7 cell lysate
Lane 3: Rabbit IgG instead of ET1609-51 in MCF7 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 23 seconds; ECL: K1801 -
Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of RAW264.7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-AKT1/2/3 antibody (ET1609-51) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-51) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/100
Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/100
Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
Flow cytometric analysis of MCF7 cells labeling AKT1/2/3.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-51, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of RAW264.7 cells labeling AKT1/2/3.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-51, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling AKT1/2/3.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-51, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Ultrasound-responsive piezoelectric hydrogels accelerate bone defect repair by regulating mitochondrial OXPHOS via activating AKT/GSK3β/β-catenin signaling axis
期刊: Chemical Engineering Journal
DOI: 10.1016/j.cej.2025.168176
IF: 13.2
应用: WB
反应种属: Mouse
发表时间: 2025 Sept
-
Bifidobacterium longum subsp. longum B-53 Alleviated Abnormalities of Glycolipid Metabolism and Oxidative Stress in T2DM Mice Through Regulating Gut Microbiota and GLP-1 Secretion
期刊: Journal Of Agricultural And Food Chemistry
DOI: 10.1021/acs.jafc.5c07346
IF: 6.2
应用: WB
反应种属: Mouse
发表时间: 2025 Sept
-
Crotalaria assamica Benth alleviated acetaminophen-induced liver injury through the upregulation of Nrf2 pathway
期刊: JOURNAL OF ETHNOPHARMACOLOGY
DOI: 10.1016/j.jep.2025.120664
IF: 5.4
应用: WB
反应种属: Mouse
发表时间: 2025 Sept
-
Multi-pathway mechanisms of Liujunzi decoction in promoting glioma apoptosis and reversing drug resistance: network pharmacology and experimental validation
期刊: Frontiers In Pharmacology
DOI: 10.3389/fphar.2025.1682550
IF: 4.8
应用: WB
反应种属: Human
发表时间: 2025 Sept
-
In vitro and clinical evaluation of platelet-rich plasma combined with angioplasty in diabetic foot treatment
期刊: World Journal of Diabetes
DOI: 10.4239/wjd.v16.i10.110631
IF: 4.6
应用: WB
反应种属: Human
发表时间: 2025 Oct
-
7,8-Dihydroxyflavone protects acetaminophen induced liver injury through activating PI3K/Akt/NRF2/GPX4 mediated ferroptosis suppression
期刊: Free Radical Biology And Medicine
DOI: 10.1016/j.freeradbiomed.2025.10.297
IF: 8.2
应用: WB
反应种属: Human
发表时间: 2025 Oct
-
ATP6V0D2 drives triple-negative breast cancer progression and inhibits cisplatin sensitivity by promoting PHLPP2 lysosomal degradation
期刊: iScience
DOI: 10.1016/j.isci.2025.113853
IF: 4.1
应用: WB
反应种属: Human
发表时间: 2025 Oct
-
Isorhamnetin Exhibits Hypoglycemic Activity and Targets PI3K/AKT and COX-2 Pathways in Type 1 Diabetes
期刊: Nutrients
DOI: 10.3390/nu17203201
IF: 5
应用: WB
反应种属: Mouse,Rat
发表时间: 2025 Oct
-
OpiCa1 Modulates Cardiomyocyte Viability Through PI3K/Akt Inhibition with Minimal Systemic Impact Beyond RyR Targeting
期刊: Toxins
DOI: 10.3390/toxins17110550
IF: 4
应用: WB
反应种属: Mouse,Rat
发表时间: 2025 Nov
-
Paeoniflorin Alleviates LPS-Induced Inflammation and Acute Myocardial Injury by Inhibiting PI3K/Akt/ERK-Mediated HIF-1α and TLR4/MyD88/NF-κB Inflammatory Signaling
期刊: Mediators of Inflammation
DOI: 10.1155/mi/2346163
IF: 4.2
应用: WB
反应种属: Rat
发表时间: 2025 Nov
-
Dual interference with host neuropeptide signaling allows parasitoid wasp to hijack host sugar metabolism
期刊: EMBO Journal
DOI: 10.1038/s44318-025-00636-5
IF: 8.3
应用: WB
反应种属: Plutella xylostella
发表时间: 2025 Nov
-
CUEDC1 promotes glycolytic metabolism reprogramming through the CUEDC1/CACNG4/PI3K axis to promote ER-positive breast cancer growth
期刊: Cellular & Molecular Biology Letters
DOI: 10.1186/s11658-025-00819-2
IF: 10.2
应用: WB
反应种属: Human,Mouse
发表时间: 2025 Nov
-
Sensory neuron TRPV1-mediated macrophage polarization and immune response regulate dental implant osseointegration
期刊: Tissue & Cell
DOI: 10.1016/j.tice.2025.103243
IF: 2.5
应用: WB
反应种属: Mouse
发表时间: 2025 Nov
-
Metalloporphyrin organic framework oxygen-generators enable tumour-targeted photodynamic therapy and metabolic reprogramming for enhanced glioblastoma treatment
期刊: Journal of Colloid and Interface Science
DOI: 10.1016/j.jcis.2025.139521
IF: 9.7
应用: WB
反应种属: Human
发表时间: 2025 Nov
-
Ultra-Small Coordination Dihydromyricetin Nanodots Attenuate Alveolar Bone Resorption in Periodontitis Through Inhibition of ITGB3-Driven Osteoclast Differentiation
期刊: Materials Today Bio
DOI: 10.1016/j.mtbio.2025.102478
IF: 10.2
应用: WB
反应种属: Mouse
发表时间: 2025 Nov
-
Highly Soluble Mussel Foot Protein Enhances Antioxidant Defense and Cytoprotection via PI3K/Akt and Nrf2/HO-1 Pathways
期刊: Antioxidants
DOI: 10.3390/antiox14060644
IF: 6
应用: WB
反应种属: Mouse
发表时间: 2025 May
-
Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44–AKT–CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation
期刊: Research
DOI: 10.34133/research.0690
IF: 8.5
应用: WB
反应种属: Mouse
发表时间: 2025 May
-
Moderate intensity continuous training mitigates hypertension-induced renal fibrosis by inhibiting HIF-1α-mediated autophagy
期刊: Frontiers In Physiology
DOI: 10.3389/fphys.2025.1529811
IF: 3.2
应用: WB
反应种属: Rat
发表时间: 2025 Mar
-
Huafengdan Inhibits Glioblastoma Cell Growth and Mobility by Acting on PLAU and CAV1 Targets
期刊: Pharmaceuticals
DOI: 10.3390/ph18030428
IF: 4.3
应用: WB
反应种属: Human
发表时间: 2025 Mar
-
Trimethylamine N-oxide (TMAO) treatment triggers premature ovarian insufficiency (POI) via the activation of mitochondrial pathway apoptosis in granulosa cells
期刊: FREE RADICAL BIOLOGY AND MEDICINE
DOI: 10.1016/j.freeradbiomed.2025.03.007
IF: 8.2
应用: WB
反应种属: Mouse
发表时间: 2025 Mar
-
A novel PEGylated squid-derived protamine protein in combination with sorafenib effectively inhibits hepatocellular carcinoma growth
期刊: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.145283
IF: 8.5
应用: WB
反应种属: Mouse
发表时间: 2025 Jun
-
Biomaterial-Based Lymphangiogenesis-Enhanced Therapeutic Strategy for Robustly-Integrated Medication-Related Osteonecrosis of Jaw Prevention
期刊: ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.5c02812
IF: 8.2
应用: WB
反应种属: Human
发表时间: 2025 Jun
-
POLR2H Serves as a Novel Prognostic Biomarker Correlated with Immune Infiltration in Prostate Cancer
期刊: Biochemical Genetics
DOI: 10.1007/s10528-025-11150-y
IF: 1.6
应用: WB
反应种属: Human
发表时间: 2025 Jun
-
High mobility group box 1, a novel serotonin receptor-7 negative modulator, contributes to M2 microglial ferroptosis and neuroinflammation in post-stroke depression
期刊: Free Radical Biology And Medicine
DOI: 10.1016/j.freeradbiomed.2025.06.025
IF: 8.2
应用: WB
反应种属: Mouse
发表时间: 2025 Jun
-
Exploring SERTAD4 as a prognostic biomarker and therapeutic target in breast cancer: insights from multidatabase analyses and in vitro studies
期刊: Clinical And Experimental Medicine
DOI: 10.1007/s10238-025-01792-y
IF: 3.5
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
Probiotic efficacy of Cetobacterium somerae (CGMCC No. 28843): promoting intestinal digestion, absorption, and structural integrity in juvenile grass carp (Ctenopharyngodon idella)
期刊: Journal of Animal Science and Biotechnology
DOI: 10.1186/s40104-025-01224-7
IF: 6.5
应用: WB
反应种属: Fish
发表时间: 2025 Jul
-
Quinolinone Derivatives Suppress Angiogenesis in Human Umbilical Vein Endothelial Cells by Blocking the VEGF-Induced VEGFR2 Signaling Pathway
期刊: Archiv Der Pharmazie
DOI: 10.1002/ardp.70040
IF: 3.6
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
Ursolic and oleanolic acids suppress MNNG induced malignant transformation of human gastric mucosal epithelium by regulating the PI3 K/AKT pathway
期刊: Scientific Reports
DOI: 10.1038/s41598-025-03606-3
IF: 3.9
应用: WB
反应种属: Human
发表时间: 2025 Jul
-
Gestational diabetes mellitus-derived miR-7-19488 targets PIK3R2 mRNA to stimulate the abnormal development and maturation of offspring-islets
期刊: Life Sciences
DOI:
IF: 5.2
应用: WB
反应种属: Rat
发表时间: 2025 Jan
-
STING1 targets MYH9 to drive adipogenesis through the AKT/GSK3β/β-catenin pathway
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI: 10.1016/j.bbrc.2025.151352
IF: 2.2
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
-
Serum Pharmacochemistry and Network Pharmacology Reveal Active Compounds and Mechanisms of the Huaxian Formula in Alleviating Radiation-Induced Pulmonary Fibrosis
期刊: Drug Design Development and Therapy
DOI: 10.2147/DDDT.S490844
IF: 5.1
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
-
NACC1 accelerates the progression of AML by regulating the ADAM9/PI3K/AKT axis
期刊: International Journal Of Medical Sciences
DOI:
IF: 3.2
应用: WB
反应种属: Human
发表时间: 2025 Jan
-
NACC1 accelerates the progression of AML by regulating the ADAM9/PI3K/AKT axis
期刊: International Journal of Medical Sciences
DOI: 10.7150/ijms.102266
IF: 3.2
应用: WB
反应种属: Human
发表时间: 2025 Jan
-
STING1 targets MYH9 to drive adipogenesis through the AKT/GSK3β/β-catenin pathway
期刊: Biochemical And Biophysical Research Communications
DOI:
IF: 2.5
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
-
Gestational diabetes mellitus-derived miR-7-19488 targets PIK3R2 mRNA to stimulate the abnormal development and maturation of offspring-islets
期刊: LIFE SCIENCES
DOI: 10.1016/j.lfs.2025.123369
IF: 5.1
应用: WB
反应种属: Rat
发表时间: 2025 Jan
-
Total flavonoids extracted from the leaves of Murraya paniculata (L.) Jack prevents acetaminophen-induced liver injury by activating Keap1/Nrf2 and PI3K/AKT/mTOR signaling pathway
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.119562
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2025 Feb
-
Betulinic Acid Inhibits Glioma Progression by Inducing Ferroptosis Through the PI3K/Akt and NRF2/HO-1 Pathways
期刊: Journal Of Gene Medicine
DOI: 10.1002/jgm.70011
IF: 3.2
应用: WB
反应种属: Human
发表时间: 2025 Feb
-
Isorhamnetin Alleviates Inflammation-Induced Crosstalk between Kynurenine Pathway and Gut Microbiota in Depressed Mice
期刊: Biomolecules & Therapeutics
DOI: 10.4062/biomolther.2024.061
IF: 3
应用: WB
反应种属: Mouse
发表时间: 2025 Feb
-
Integrating network pharmacology and experimental validation to explore the mechanism of Yiqi Ziyin in treating immune thrombocytopenia
期刊: Scientific Reports
DOI: 10.1038/s41598-025-14105-w
IF: 3.9
应用: WB
反应种属: Mouse
发表时间: 2025 Aug
-
HIF-1α regulates the proliferation and differentiation of mouse cranial base sphenoid-occipital synchondrosis chondrocytes via PI3K/Akt signaling
期刊: Scientific Reports
DOI: 10.1038/s41598-025-11055-1
IF: 3.9
应用: WB
反应种属: Mouse
发表时间: 2025 Aug
-
Microenvironment-adaptive nanomedicine MXene promotes flap survival by inhibiting ROS cascade and endothelial pyroptosis
期刊: Journal Of Nanobiotechnology
DOI: 10.1186/s12951-025-03343-9
IF: 10.6
应用: WB
反应种属: Mouse
发表时间: 2025 Apr
-
Loss of lims1 causes aberrant cardiac remodeling and heart failure via activating gp130/Jak1/Stat3 pathway in zebrafish
期刊: Journal of Genetics and Genomics
DOI: 10.1016/j.jgg.2025.04.003
IF: 6.6
应用: WB
反应种属: Zebrafish
发表时间: 2025 Apr
-
Enhanced migration and adhesion protein expression by polyethylene glycol 4-modified SVVYGLR peptide in an in vitro human gingival fibroblast wound healing model
期刊: Folia Histochemica Et Cytobiologica
DOI: 10.5603/fhc.104010
IF: 1.7
应用: WB
反应种属: Mouse
发表时间: 2025 Apr
-
CDCP1 knockdown suppresses PDGFRβ/AKT pathway-mediated vascular smooth muscle cell proliferation by inhibiting PDGFRβ endocytosis
期刊: Peer J
DOI: 10.7717/peerj.19114
IF: 2.3
应用: WB
反应种属: Mouse
发表时间: 2025 Apr
-
Quercetin inhibited LPS-induced cytokine storm by interacting with the AKT1-FoxO1 and Keap1-Nrf2 signaling pathway in macrophages
期刊: Scientific Reports
DOI: 10.1038/s41598-024-71569-y
IF: 3.8
应用: WB
反应种属: Mouse
发表时间: 2024 Sept
-
Genipin promotes the apoptosis and autophagy of neuroblastoma cells by suppressing the PI3K/AKT/mTOR pathway
期刊: Scientific Reports
DOI:
IF: 3.8
应用: WB
反应种属: Human
发表时间: 2024 Sep
-
Framework Nucleic Acids Loaded with Quercetin: Protecting Retinal Neurovascular Unit via the Protein Kinase B/Heme Oxygenase-1 Pathway
期刊: ACS Applied Nano Materials
DOI:
IF: 15.8
应用: IF,WB
反应种属: Mouse
发表时间: 2024 Sep
-
Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer
期刊: Molecular Cancer
DOI:
IF: 27.7
应用: WB
反应种属: Human
发表时间: 2024 Oct
-
Chaperonin-containing TCP1 subunit 6A inhibition via TRIM21-mediated K48-linked ubiquitination suppresses triple-negative breast cancer progression through the AKT signalling pathway
期刊: Clinical And Translational Medicine
DOI: 10.1002/ctm2.70097
IF: 7.9
应用: WB
反应种属: Human
发表时间: 2024 Nov
-
Exploring the Osteogenic Effects of Simiao Wan through Activation of the PI3K/AKT Pathway in Osteoblasts
期刊: Journal Of Ethnopharmacology
DOI:
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2024 Nov
-
Resveratrol alleviates depression-like behaviors by inhibiting ferroptosis via AKT/NRF2 pathway
期刊: Brain Research Bulletin
DOI:
IF: 3.5
应用: WB
反应种属: Rat
发表时间: 2024 Nov
-
Shikonin protects mitochondria through the NFAT5/AMPK pathway for the treatment of diabetic wounds
期刊: World Journal Of Diabetes
DOI:
IF: 4.2
应用: WB
反应种属: Human
发表时间: 2024 Nov
-
Identification of SARS-CoV-2-specific T cell and its receptor
期刊: Journal Of Hematology & Oncology
DOI:
IF: 29.5
应用: WB
反应种属: Human
发表时间: 2024 Mar
-
SORBS1 inhibits epithelial to mesenchymal transition (EMT) of breast cancer cells by regulating PI3K/AKT signaling and macrophage phenotypic polarization
期刊: Aging-Us
DOI: 10.18632/aging.205632
IF: 3.9
应用: WB
反应种属: Human
发表时间: 2024 Mar
-
Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment
期刊: Advanced Functional Materials
DOI:
IF: 19.9
应用: WB
反应种属: Human
发表时间: 2024 Jun
-
Extracellular Vesicles Containing GAS6 Protect the Liver from Ischemia-Reperfusion Injury by Enhancing Macrophage Efferocytosis via MerTK-ERK-COX2 Signaling
期刊: Preprint And Has Not Been Certified By Peer Review
DOI:
IF:
应用: WB
反应种属: Mouse
发表时间: 2024 Jun
-
A novel L-shaped ortho-quinone analog suppresses glioblastoma progression by targeting acceleration of AR degradation and regulating PI3K/AKT pathway
期刊: Biochemical Pharmacology
DOI: 10.1016/j.bcp.2024.116398
IF: 5.3
应用: WB
反应种属: Human
发表时间: 2024 Jun
-
Inhibiting PRMT1 protects against CoNV by regulating macrophages through the FGF2/PI3K/Akt pathway
期刊: European Journal Of Pharmacology
DOI:
IF: 5
应用: WB
反应种属: Mouse
发表时间: 2024 Jun
-
Disintegrin Accutin inhibits A549 cell migration though suppression of EMT and FAK/AKT signaling pathway
期刊: International Journal Of Biological Macromolecules
DOI:
IF: 7.7
应用: WB
反应种属: Human
发表时间: 2024 Jul
-
The Therapeutic Potential of Chi Shao and Fu Zi in Acute-on-Chronic Liver Failure: Insights from Network Pharmacology and Animal Studies
期刊: Preprint And Has Not Been Certified By Peer Review
DOI:
IF:
应用: WB
反应种属: Rat
发表时间: 2024 Jul
-
HNRNP I promotes IRAK1 degradation to reduce podocyte apoptosis and inflammatory response alleviating renal injury in diabetic nephropathy
期刊: Immunobiology
DOI:
IF: 2.5
应用: WB
反应种属: Human
发表时间: 2024 Jul
-
Enhancing the therapeutic efficacy of gefitinib on subcutaneously transplanted SKOV3 ovarian cancer tumors in nude mice via ultrasound‑stimulated microbubble cavitation
期刊: Experimental And Therapeutic Medicine
DOI:
IF: 2.4
应用: WB
反应种属: Mouse
发表时间: 2024 Jul
-
Effect of Sonic hedgehog gene-modified bone marrow mesenchymal stem cells on graft-induced retinal gliosis and retinal ganglion cells survival in diabetic mice
期刊: International Journal Of Ophthalmology
DOI: 10.18240/ijo.2024.01.05
IF: 1.9
应用: WB
反应种属: Mouse
发表时间: 2024 Jan
-
Hyperuricemia Induces Reproductive Damage in Male Rats through Zinc Homeostasis Imbalance and Oxidative Stress
期刊: Andrology
DOI:
IF: 2.5
应用: WB
反应种属: Rat
发表时间: 2024 Jan
-
Novel inhibitors targeting the PGK1 metabolic enzyme in glycolysis exhibit effective antitumor activity against kidney renal clear cell carcinoma in vitro and in vivo
期刊: European Journal Of Medicinal Chemistry
DOI: 10.1016/j.ejmech.2024.116209
IF: 6
应用: WB
反应种属: Human
发表时间: 2024 Feb
-
Sulforaphane inhibits multiple myeloma cell-induced osteoclast differentiation and macrophage proliferation by elevating ferroportin1
期刊: Cancer Chemotherapy And Pharmacology
DOI:
IF: 2.7
应用: WB
反应种属: Human
发表时间: 2024 Dec
-
Regulation of Glycolysis-Derived L-lactate Production in Astrocytes Rescues the Memory Deficits and Aβ Burden in Early Alzheimer's Disease Models
期刊: Pharmacological Research
DOI:
IF: 9.1
应用: WB
反应种属: Mouse
发表时间: 2024 Aug
-
Rhein‐based Pickering emulsion for hepatocellular carcinoma: Shaping the metabolic signaling and immunoactivation in transarterial chemoembolization
期刊: Aggregate
DOI:
IF: 18.8
应用: WB
反应种属: Human
发表时间: 2024 Apr
-
Exosome/antimicrobial peptide laden hydrogel wound dressings promote scarless wound healing through miR-21-5p-mediated multiple functions
期刊: Biomaterials
DOI:
IF: 14
应用: WB
反应种属: Human,Mouse
发表时间: 2024 Apr
-
Enhancing metformin-induced tumor metabolism destruction by glucose oxidase for triple-combination therapy
期刊: Journal Of Pharmaceutical Analysis
DOI: 10.1016/j.jpha.2023.09.015
IF: 8.8
应用: WB
反应种属: Mouse
发表时间: 2023 Sept
-
LncRNA RP11-93B14. 5 promotes gastric cancer cell growth through PI3K/AKT signaling pathway
期刊: Molecular Biotechnology
DOI:
IF: 2.6
应用: WB
反应种属: Human
发表时间: 2023 Sept
-
Antibacterial and Angiogenic (2A) Bio-Heterojunctions Facilitate Infectious Ischemic Wound Regeneration via an Endogenous-Exogenous Bistimulatory Strategy
期刊: Advanced Materials
DOI:
IF: 29.4
应用: WB
反应种属: Mouse
发表时间: 2023 Oct
-
dCas9-Based PDGFR-β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling
期刊: International Journal Of Molecular Sciences
DOI:
IF: 6.208
应用: WB
反应种属: Mouse,Human
发表时间: 2023 Mar
-
GLP‑1 receptor agonist protects palmitate-induced insulin resistance in skeletal muscle cells by up-regulating sestrin2 to promote autophagy
期刊: Scientific Reports
DOI:
IF: 4.996
应用: WB
反应种属: Rat
发表时间: 2023 Jun
-
Mechanisms of action underlying the effect of Tongsaimai on wound healing based on experimental and network pharmacology
期刊: Journal Of Ethnopharmacology
DOI:
IF: 5.195
应用: WB
反应种属: Mouse
发表时间: 2023 Jul
-
Upregulation of glutamate transporter 1 by mTOR/Akt pathway in astrocyte culture during oxygen-glucose deprivation and reoxygenation
期刊: Experimental Brain Research
DOI:
IF: 2
应用: WB
反应种属: Rat
发表时间: 2023 Jan
-
Tetrahedral framework nucleic acids inhibit pathological neovascularization and vaso-obliteration in ischaemic retinopathy via PI3K/AKT/mTOR signalling pathway
期刊: Cell Proliferation
DOI:
IF:
应用: WB
反应种属: Human
发表时间: 2023 Jan
-
Carvedilol exhibits anti-acute T lymphoblastic leukemia effect in vitro and in vivo via inhibiting β-ARs signaling pathway
期刊: Biochemical And Biophysical Research Communications
DOI:
IF: 3.1
应用: WB
反应种属: Human
发表时间: 2023 Jan
-
Icariin improves learning and memory function in Aβ1-42-induced AD mice through regulation of the BDNF-TrκB signaling pathway
期刊: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2023.117029
IF: 5.4
应用: WB
反应种属: Mouse
发表时间: 2023 Aug
-
Circular RNA hsa_circ_0084054 promotes the progression of periodontitis with diabetes via the miR‐508‐3p/PTEN axis
期刊: Journal Of Periodontal Research
DOI:
IF: 3.5
应用: WB
反应种属: Human
发表时间: 2023 Aug
-
Downregulation of fibulin-4 inhibits autophagy and promotes the sensitivity of esophageal squamous cell carcinoma cells to apatinib by activating the Akt-mTOR signaling pathway
期刊: Thoracic Cancer
DOI:
IF: 3.223
应用: WB
反应种属: Human
发表时间: 2022 Sept
-
Ozone causes depressive-like response through PI3K/Akt/GSK3β pathway modulating synaptic plasticity in young rats
期刊: Ecotoxicology And Environmental Safety
DOI: 10.1016/j.ecoenv.2022.114171
IF: 7.129
应用: WB
反应种属: Rat
发表时间: 2022 Oct
-
Icariin alleviates osteoarthritis through PI3K/Akt/mTOR/ULK1 signaling pathway
期刊: European Journal Of Medical Research
DOI:
IF: 4.981
应用: WB
反应种属: Human
发表时间: 2022 Oct
-
Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens
期刊: Cells
DOI:
IF: 7.666
应用: WB
反应种属: Chicken
发表时间: 2022 Oct
-
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
期刊: Molecular Cancer
DOI:
IF: 41.444
应用: WB
反应种属: Human
发表时间: 2022 May
-
Estradiol reduced 5-HT reuptake by downregulating the gene expression of Plasma Membrane Monoamine Transporter (PMAT, Slc29a4) through estrogen receptor β and the MAPK/ERK signaling pathway
期刊: European Journal Of Pharmacology
DOI:
IF: 4.432
应用: WB
反应种属: Rat
发表时间: 2022 Jun
-
PD-L1 regulates cell proliferation and apoptosis in acute myeloid leukemia by activating PI3K-AKT signaling pathway
期刊: Scientific Reports
DOI:
IF: 4.380
应用: WB
反应种属: Human
发表时间: 2022 Jul
-
PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis
期刊: Cell Death & Disease
DOI:
IF: 8.469
应用: WB
反应种属: Human
发表时间: 2022 Feb
-
Ginsenoside Rk1 Prevents UVB Irradiation-Mediated Oxidative Stress, Inflammatory Response, and Collagen Degradation via the PI3K/AKT/NF-κB Pathway In Vitro and In Vivo
期刊: Journal Of Agricultural And Food Chemistry
DOI:
IF: 6.1
应用: WB
反应种属: Human
发表时间: 2022 Dec
-
Nickel nanoparticles affect the migration and invasion of HTR-8/SVneo cells by downregulating MMP2 through the PI3K/AKT pathway
期刊: Toxicology In Vitro
DOI:
IF: 3.2
应用: WB
反应种属: Human
发表时间: 2022 Apr
-
Aligned fibrin/functionalized self-assembling peptide interpenetrating nanofiber hydrogel presenting multi-cues promotes peripheral nerve functional recovery
期刊: Bioactive Materials
DOI:
IF: 14.594
应用: WB
反应种属: Rat
发表时间: 2021 Jun
-
Uncovering the mechanisms of leech and centipede granules in the treatment of diabetes mellitus-induced erectile dysfunction utilising network pharmacology
期刊: Journal Of Ethnopharmacology
DOI:
IF: 5.4
应用: WB
反应种属: Rat
发表时间: 2021 Jan
-
Tumor Suppressor Functions of miRNA-375 in Nasopharyngeal Carcinoma through Inhibition of Ubiquitin-Specific Protease 1 Expression
期刊: International Journal Of Biochemistry And Cell Biology
DOI:
IF: 5.09
应用: WB
反应种属: Human
发表时间: 2021 Dec
-
Inhibition of the activation of γδT17 cells throughPPARγ–PTEN/Akt/GSK3β/NFAT pathwaycontributes to the anti-colitis effect of madecassicacid
期刊: Cell Death & Disease
DOI:
IF: 6.30
应用: WB
反应种属: Mouse
发表时间: 2020 Sept
-
Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways
期刊: Oxidative Medicine And Cellular Longevity
DOI:
IF: 5.076
应用: WB,IF,IHC-P
反应种属: Chicken
发表时间: 2020 Nov
-
CD44 inhibition attenuates EGFR signaling and enhances cisplatin sensitivity in human EGFR wild-type non-small-cell lung cancer cells
期刊: International Journal Of Molecular Medicine
DOI:
IF: 2.928
应用: WB,IF
反应种属: Human
发表时间: 2020 Jun
-
Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3
期刊: Cell Death & Disease
DOI:
IF: 5.64
应用: WB
反应种属: Human
发表时间: 2019 Feb
-
Knockdown of ISOC1 inhibits the proliferation and migration and induces the apoptosis of colon cancer cells through the AKT/GSK-3β pathway
期刊: Carcinogenesis
DOI:
IF: 4.004
应用: WB
反应种属: Human
发表时间: 2019 Aug
同靶点 & 同通路的产品
AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [JE75-09] - BSA and Azide free
Application: WB,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [JE75-09]
Application: WB,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat,Monkey
Conjugate: unconjugated
浙公网安备 33019202000643号