图片
-
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601082) at 1/40,000 dilution.
Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: THP-1 cell lysate (10 µg/Lane)
Lane 4: PMVEC cell lysate (10 µg/Lane)
Lane 5: RH-35 cell lysate (10 µg/Lane)
Lane 6: NIH/3T3 cell lysate (10 µg/Lane)
Lane 7: RAW264.7 cell lysate (10 µg/Lane)
Lane 8: B16F1 cell lysate (10 µg/Lane)
Lane 9: MEF cell lysate (10 µg/Lane)
Lane 10: Hela cell lysate (10 µg/Lane)
Lane 11: F9 cell lysate (10 µg/Lane)
Lane 12: PC-12 cell lysate (10 µg/Lane)
Lane 13: Mouse lung cell lysate (20 µg/Lane)
Lane 14: L6 cell lysate (10 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
-
Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
-
Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
-
Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"