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HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

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Catalog# ET1702-67

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

  • Monkey

  • Hamster

  • Arabidopsis thaliana

  • Rice

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant full length protein of Human beta Actin aa 1-375 / 375.

种属反应性

Human, Mouse, Rat, Zebrafish, Monkey, Hamster, Arabidopsis thaliana, Rice

验证应用

WB

分子量

Predicted band size: 42 kDa

阳性对照

HeLa cell lysate, A549 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, hybrid fish (crucian-carp) brain tissue lysates.

偶联

HRP

克隆号

JF53-10

RRID

AB_2890197

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:10,000

发表文章中的应用

WB See 21 publications below
IF See 1 publications below

靶点

功能

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

背景文献

1. Wang H et al. Role of the orexin (hypocretin) system in contextual fear conditioning in rats. Behav Brain Res 316:47-53 (2017).

2. Gruber R et al. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK-STAT3 Signaling. Gastroenterology 151:526-39 (2016).

序列相似性

Belongs to the actin family.

翻译后修饰

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

亚细胞定位

Cytoplasm.

UNIPROT #

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P60711 Rat

别名

A26C1A antibody

A26C1B antibody

ACTB antibody

ACTB_HUMAN antibody

Actin beta antibody

Actin cytoplasmic 1 antibody

Actin, cytoplasmic 1, N-terminally processed antibody

Actx antibody

b actin antibody

Beta cytoskeletal actin antibody

展开

图片

  • Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (<a href="/products/ET1702-67" style="font-weight: bold;text-decoration: underline;">ET1702-67</a>) at 1/10,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: PC-12 cell lysate<br />Lane 6: Mouse brain tissue lysate<br />Lane 7: Rat brain tissue lysate<br />Lane 8: Mouse lung tissue lysate<br />Lane 9: Rat lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-67" style="font-weight: bold;text-decoration: underline;">ET1702-67</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (ET1702-67) at 1/10,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: PC-12 cell lysate
    Lane 6: Mouse brain tissue lysate
    Lane 7: Rat brain tissue lysate
    Lane 8: Mouse lung tissue lysate
    Lane 9: Rat lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-67) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-67" style="font-weight: bold;text-decoration: underline;">ET1702-67</a>, 1/500) was used in 5% BSA at room temperature for 2 hours.

    Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IF

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