HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
Catalog# M1210-5
HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
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WB
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Human
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Mouse
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Rat
概述
产品名称
HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within N-terminal human Beta-actin .
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
42 kDa
阳性对照
PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate.
偶联
HRP
克隆号
A2-F6
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
发表文章中的应用
发表文章中的种属
| Mouse | See 4 publications below |
| Human | See 1 publications below |
靶点
功能
Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
背景文献
1. Fujiki R et al. GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Nature 459:455-459 (2009).
亚细胞定位
Cytoplasm.
别名
A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
展开图片
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Western blot analysis of beta Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-5, 1/1,000) was used in 5% BSA at room temperature for 2 hours.
Positive control:
Lane 1: PC12 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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CXCL5 activates CXCR2 in nociceptive sensory neurons to drive joint pain and inflammation in experimental gouty arthritis
Author: Yin Chengyu,et al
PMID: 38627393
应用: WB
反应种属: Mouse
发表时间: 2024 Apr
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Citation
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Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel
Author: Zhao, W., He, X., Liu, R., & Ruan, Q.
PMID: 36703231
应用: WB
反应种属: Mouse
发表时间: 2023 Jan
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Citation
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MYCN protein stability is a better prognostic indicator in neuroblastoma
Author:
PMID: 35820898
应用: WB
反应种属: Human
发表时间: 2022 Jul
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Citation
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A. Muciniphila Suppresses Colorectal Tumorigenesis by Inducing TLR2/NLRP3-Mediated M1-Like TAMs
Author:
PMID: 34389559
应用: WB
反应种属: Mouse
发表时间: 2021 Oct
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Citation
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Shp2 in myocytes is essential for cardiovascular and neointima development.
Author: Hongqiang Cheng;
PMID: 31634485
应用: WB
反应种属: Mouse
发表时间: 2019 Dec
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Citation
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