mNeonGreen Recombinant Rabbit Monoclonal Antibody [PSH19-59]
Rmb: 880 特惠 1500
Catalog# HA724085
mNeonGreen Recombinant Rabbit Monoclonal Antibody [PSH19-59]
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IF-Tissue
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WB
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IP
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Species independent
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unconjugated
概述
产品名称
mNeonGreen Recombinant Rabbit Monoclonal Antibody [PSH19-59]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Common lancelet mNeonGreen aa 1-260.
种属反应性
Species independent
验证应用
IF-Tissue, WB, IP
靶点分子量
Predicted band size: 29 kDa
偶联
unconjugated
克隆号
PSH19-59
反应性数据
已验证（内部验证通过）
文献已报道（未内部验证，但有文献支持）
预测可反应（基于高同源性）
不推荐（内部验证未通过）
| WB | IHC-P | FC | IF-Cell | IF-Tissue | IP | |
|---|---|---|---|---|---|---|
| species independent |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Tissue
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1:2,000
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WB
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1:5,000
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IP
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1-2μg/sample
靶点
功能
The fluorescent proteins from lancelets have been adapted for use in molecular biology and microscopy. The yellow fluorescent protein from Branchiostoma lanceolatum exhibits unusually high quantum yield (~0.95). It has been engineered into a monomeric green fluorescent protein known as mNeonGreen, which is the brightest known monomeric green or yellow fluorescent protein.
背景文献
1. Joshi M et al. mNeonGreen aggregates when overexpressed in C. elegans neurons. MicroPubl Biol. 2024 Feb
2. Hoshizaki J et al. Efficient generation of mNeonGreen Plasmodium falciparum reporter lines enables quantitative fitness analysis. Front Cell Infect Microbiol. 2022 Sep
别名
mNeonGreen
图片
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Sample: Mouse cartilage
Green: Gene X-mNeonGreen;
Red: anti-mNeonGreen (HA724085);
Blue: Hoechst
The far left is the merged fluorescence image. -
Western blot analysis of mNeonGreen on different lysates with Rabbit anti-mNeonGreen antibody (HA724085) at 1/5,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with FLAG-tagged mNeonGreen cell lysate
Lane 3: 293T transfected with GFP cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724085) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
mNeonGreen was immunoprecipitated from 0.2 mg 293T transfected with FLAG-tagged mNeonGreen cell lysate with HA724085 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724085 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with FLAG-tagged mNeonGreen cell lysate (input)
Lane 2: HA724085 IP in 293T transfected with FLAG-tagged mNeonGreen cell lysate
Lane 3: Rabbit IgG instead of HA724085 in 293T transfected with FLAG-tagged mNeonGreen cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 2 seconds; ECL: K1801
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