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mAID tag Rabbit Polyclonal Antibody

RMB: 599 特惠 1500

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Catalog# ER1802-93

mAID tag Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Species independent

概述

产品名称

mAID tag Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide immune sequence is PKDPAKPPAKAQVVGWPC.

种属反应性

Species independent

验证应用

WB

阳性对照

Mini-AID-tagged fusion protein.

偶联

unconjugated

RRID

AB_3069193

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

靶点

功能

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

背景文献

1. Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171(7):1692-1706 (2017).

图片

  • Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.

    Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"