HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control
Rmb: 100 特惠 150
Catalog# HA601585H
HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control
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WB
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Human
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Mouse
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Rat
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Green monkey
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Pig
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HRP
概述
产品名称
HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Synthetic peptide within N-terminal human Beta-actin .
种属反应性
Human, Mouse, Rat, Green monkey, Pig
验证应用
WB
分子量
Predicted band size: 42 kDa
阳性对照
Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Pig brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.
偶联
HRP
克隆号
A2-F6-R
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000-1:5,000
靶点
功能
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
背景文献
1. Fujiki R et al. GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Nature 459:455-459 (2009).
亚细胞定位
Cytoplasm.
别名
A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
展开图片
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Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat liver tissue lysate
Lane 4: Pig brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour. -
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour. -
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/5,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 3: Rat lung tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 4: Rat colon tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/5,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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