VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]
Catalog# ET1607-74
VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
VPS34 Recombinant Rabbit Monoclonal Antibody [SY0286]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human VPS34 aa 830-870.
种属反应性
Human, Mouse, Rat
验证应用
IHC-P, FC
分子量
Predicted band size: 102 kDa
阳性对照
Human stomach cancer tissue, mouse brain tissue, rat brain tissue, A549.
偶联
unconjugated
克隆号
SY0286
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:50-1:200
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FC
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1:1,000
靶点
功能
Phosphatidylinositol 3-kinase catalytic subunit type 3 is an enzyme subunit that in humans is encoded by the PIK3C3 gene. It's a class III phosphoinositide 3-kinase. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. PI 3-kinase p100 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. PI 3-kinase p100 exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. Unlike class I PI3Ks, whose activities are enhanced in the presence of magnesium, PI 3-kinase p100 activity is enhanced by manganese. Its activity can also be regulated by nutrients, suggesting an important role of PI-3 kinase p100 in the regulation of mTOR protein synthesis and autophagy.
背景文献
1. Bechtel W et al.2013. Vps34 deficiency reveals the importance of endocytosis for podocyte homeostasis. J Am Soc Nephrol 24:727-43.
2. Liu, MN. et al. 2015. Functional mechanism of the enhancement of 5-fluorouracil sensitivity by TUSC4 in colon cancer cells. Oncol Lett. 10: 3682-3688.
序列相似性
Belongs to the PI3/PI4-kinase family.
组织特异性
Ubiquitously expressed, with a highest expression in skeletal muscle.
亚细胞定位
Midbody, Late endosome, Cytoplasmic vesicle.
别名
hVps34 antibody
MGC61518 antibody
Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
Phosphatidylinositol 3 kinase class 3 antibody
Phosphatidylinositol 3 kinase p100 subunit antibody
Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
Phosphatidylinositol 3-kinase p100 subunit antibody
Phosphoinositide 3 kinase class 3 antibody
Phosphoinositide-3-kinase class 3 antibody
PI3 kinase type 3 antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VPS34 antibody (ET1607-74) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VPS34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of A549 cells labeling VPS34.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1607-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Hespintor Negative Regulation of PI3K/Akt Pathway Induces Cell Cycle Arrest of Hepatocellular Carcinoma
期刊: Bulletin Of Experimental Biology And Medicine
DOI:
IF: 0.9
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
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Hespintor Negative Regulation of PI3K/Akt Pathway Induces Cell Cycle Arrest of Hepatocellular Carcinoma
期刊: BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
DOI: 10.1007/s10517-025-06314-0
IF: 0.6
应用: WB
反应种属: Mouse
发表时间: 2025 Jan
同靶点 & 同通路的产品
VPS34 Recombinant Rabbit Monoclonal Antibody [PSH10-64] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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