Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free
Catalog# HA750333
Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free
-
WB
-
IP
-
FC
-
Human
-
Mouse
-
Rat
-
ET1702-06
含抗保成分
-
unconjugated
概述
产品名称
Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD71 aa 22-60 / 760.
种属反应性
Human, Mouse, Rat
验证应用
WB, IP, FC
分子量
Predicted band size: 85 kDa
阳性对照
HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.
偶联
unconjugated
克隆号
JF0956
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:10,000
-
FC
-
1:1,000
-
IP
-
Use at an assay dependent concentration.
靶点
功能
CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.
背景文献
1. Xiong L et al. Nutrition impact on ILC3 maintenance and function centers on a cell-intrinsic CD71-iron axis. Nat Immunol. 2023 Oct;
2. Chiappelli F. CD71: Role in permafrost immunity. Bioinformation. 2024 Mar
序列相似性
Belongs to the peptidase M28 family. M28B subfamily.
翻译后修饰
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
亚细胞定位
Cell membrane, Melanosome, Secreted.
别名
CD 71 antibody
CD71 antibody
CD71 antigen antibody
IMD46 antibody
OTTHUMP00000208523 antibody
OTTHUMP00000208524 antibody
OTTHUMP00000208525 antibody
p90 antibody
sTfR antibody
T9 antibody
展开图片
-
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: SW480 cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/1,000 dilution.
Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si Transferrin Receptor cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 1 minute 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (HA750333) at 1/5,000 dilution.
Lane 1: HAP1 WT cell lysate
Lane 2: HAP1 Transferrin Receptor KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 90 kDa
Exposure time: 1 minute 48 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750333) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71).
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750333, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [PSH13-56]
Application: WB,IF-Cell,IHC-P,FC,IP,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Transferrin Receptor (CD71) Rabbit Polyclonal Antibody
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
浙公网安备 33019202000643号