Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] - BSA and Azide free
Rmb: 5600
Catalog# HA752007
Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
-
IF-Tissue
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Human
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Mouse
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Rat
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ET1608-50
含抗保成分
-
Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Tenascin C aa 2,152-2,201 / 2,201.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IHC-P, IHC-Fr, IF-Tissue
靶点分子量
Predicted band size: 241 kDa
阳性对照
Human tonsil tissue, mouse embryonic cartilage tissue, mouse cerebellum tissue, rat cerebellum tissue, U-87 MG cell lysates.
偶联
unconjugated
克隆号
SU36-01
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:200-1:500
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IHC-Fr
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1:200
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IF-Tissue
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1:500
靶点
功能
Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. Expression of TN-C changes from development to adulthood. TN-C is highly expressed during embryogenesis and is briefly expressed during organogenesis, while in developed organs, expression is absent or in trace amounts. In the developing central nervous system, TN-C is involved in regulating the proliferation of both oligodendrocyte precursor cells and astrocytes. The regulation of TN-C is induced or repressed by a number of different factors that are expressed during embryonic tissue, as well as developed tissues during remodeling, injured, or neoplastic.
背景文献
1. Govindarajan P et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014).
2. Su K et al. Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair. Acta Biomater 10:5012-20 (2014).
序列相似性
Belongs to the tenascin family.
亚细胞定位
Extracellular matrix.
别名
150 225 antibody
Cytotactin antibody
Glioma associated extracellular matrix antigen antibody
Glioma-associated-extracellular matrix antigen antibody
GMEM antibody
GP 150 225 antibody
GP 150-225 antibody
GP antibody
Hexabrachion antibody
HXB antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Tenascin C antibody (HA752007) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752007) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryonic cartilage tissue with Rabbit anti-Tenascin C antibody (HA752007) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752007) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Tenascin C antibody (HA752007) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752007) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Tenascin C antibody (HA752007) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752007) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Tenascin C on U-87 MG cell lysates with Rabbit anti-Tenascin C antibody (HA752007) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 241 kDa
Observed band size: 241-350 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA752007) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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