TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
Catalog# HA750950
TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
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WB
-
IF-Cell
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Human
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Rat
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HA722126
含抗保成分
-
unconjugated
概述
产品名称
TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TIMP3 aa 1-211 / 211.
种属反应性
Human, Rat
验证应用
WB, IF-Cell
分子量
Predicted band size: 24 kDa
阳性对照
HepG2 cell lysate, A431 cell lysate, rat placenta tissue lysate, rat spleen tissue lysate, HeLa, HepG2.
偶联
unconjugated
克隆号
PSH04-41
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
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1:100
靶点
功能
Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.
背景文献
1. Casagrande V et al. TIMP3 involvement and potentiality in the diagnosis, prognosis and treatment of diabetic nephropathy. Acta Diabetol. 2021 Dec
2. Guan B et al. Early-Onset TIMP3-Related Retinopathy Associated With Impaired Signal Peptide. JAMA Ophthalmol. 2022 Jul
亚细胞定位
Secreted, extracellular space, extracellular matrix.
别名
HSMRK222 antibody
K222 antibody
K222TA2 antibody
Metalloproteinase inhibitor 3 antibody
MIG 5 protein antibody
MIG5 protein antibody
Protein MIG 5 antibody
Protein MIG-5 antibody
SFD antibody
Sorsby fundus dystrophy pseudoinflammatory antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of TIMP3 on different lysates with Rabbit anti-TIMP3 antibody (HA750950) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: Human liver tissue lysate (negative)
Lane 4: Rat placenta tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750950) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA750950) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA750950) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of HepG2 cells labeling TIMP3 with Rabbit anti-TIMP3 antibody (HA750950) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TIMP3 antibody (HA750950) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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